The role of ROS toxicity in spontaneous aneuploidy in cultured cells.

It is well known that the karyotype of animal cells cultured in vitro tends to become aneuploid as the culture ages. Aneuploidy can cause genetic instability, alter the biological properties of cells, and affect their application in genetic studies and cell engineering. Understanding the causes and mechanisms of aneuploidy is primary to control its occurrence in cultured cells, and is also helpful to understand the mechanisms of tumorigenesis because aneuploidy is a hallmark of tumor cells. This review underscores the potential role of reactive oxygen species (ROS) toxicity in spontaneous aneuploidy of cultured cells. The underlying mechanisms and possible sources of ROS are also discussed.