Literature DB >> 23105827

Stability of total and free prostate specific antigen in serum submitted to intermittent cold storage conditions.

Asmahan A El Ezzi1, Mohammed A El-Saidi.   

Abstract

The goal of this study was to determine the stability of Total Prostrate Specific antigen (PSA-T) and Free Prostrate Specific Antigen (PSA-F) in archival serum stored at 4°C and -20°C and subjected to temperature shift due to interruption in power supply. Our study showed that PSA-T was stable up to 285 days and PSA-F was stable for 158 days under these conditions. Since power supply interruption is an unavoidable problem in developing nations, our study has implication on the validity of measurement of PSA-T and PSA-F in serum that was not properly stored due to emergency situations and for certain types of retrospective studies.

Entities:  

Keywords:  Immunoradiometric Assay; Interrupted power supply; PSA Free; PSA Total; Prostate SpecificAntigen; Stability

Year:  2009        PMID： 23105827      PMCID： PMC3453223          DOI： 10.1007/s12291-009-0030-1

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  19 in total

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Authors:  Peter Albers
Journal:  Eur Urol       Date:  2006-08-08       Impact factor: 20.096

2.  Storage conditions for serum for estimating prostate-specific antigen.

Authors:  B Simm; M Gleeson
Journal:  Clin Chem       Date:  1991-01       Impact factor: 8.327

3.  Ultrasensitive detection of prostate-specific antigen by a time-resolved immunofluorometric assay and the Immulite immunochemiluminescent third-generation assay: potential applications in prostate and breast cancers.

Authors:  R A Ferguson; H Yu; M Kalyvas; S Zammit; E P Diamandis
Journal:  Clin Chem       Date:  1996-05       Impact factor: 8.327

4.  Preanalytical determinants of total and free prostate-specific antigen and their ratio: blood collection and storage conditions.

Authors:  K Jung; P von Klinggräff; B Brux; P Sinha; D Schnorr; S A Loening
Journal:  Clin Chem       Date:  1998-03       Impact factor: 8.327

5.  Stability of free prostate-specific antigen in serum samples under a variety of sample collection and sample storage conditions.

Authors:  D Woodrum; C French; L B Shamel
Journal:  Urology       Date:  1996-12       Impact factor: 2.649

6.  Reproducibility and accuracy of measurements of free and total prostate-specific antigen in serum vs plasma after long-term storage at -20 degrees C.

Authors:  David Ulmert; Charlotte Becker; Jan-Ake Nilsson; Timo Piironen; Thomas Björk; Jonas Hugosson; Göran Berglund; Hans Lilja
Journal:  Clin Chem       Date:  2005-12-29       Impact factor: 8.327

7.  A complex between prostate-specific antigen and alpha 1-antichymotrypsin is the major form of prostate-specific antigen in serum of patients with prostatic cancer: assay of the complex improves clinical sensitivity for cancer.

Authors:  U H Stenman; J Leinonen; H Alfthan; S Rannikko; K Tuhkanen; O Alfthan
Journal:  Cancer Res       Date:  1991-01-01       Impact factor: 12.701

8.  Free and complexed prostate-specific antigen (PSA): in vitro stability, epitope map, and development of immunofluorometric assays for specific and sensitive detection of free PSA and PSA-alpha 1-antichymotrypsin complex.

Authors:  K Pettersson; T Piironen; M Seppälä; L Liukkonen; A Christensson; M T Matikainen; M Suonpää; T Lövgren; H Lilja
Journal:  Clin Chem       Date:  1995-10       Impact factor: 8.327

9.  Stability of serum prostate-specific antigen determination across laboratory, assay, and storage time.

Authors:  S J Jacobsen; G G Klee; H Lilja; G L Wright; J E Oesterling
Journal:  Urology       Date:  1995-03       Impact factor: 2.649

10.  Serum concentrations of prostate specific antigen and its complex with alpha 1-antichymotrypsin before diagnosis of prostate cancer.

Authors:  U H Stenman; M Hakama; P Knekt; A Aromaa; L Teppo; J Leinonen
Journal:  Lancet       Date:  1994-12-10       Impact factor: 79.321
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