| Literature DB >> 23105784 |
Rani James1, K Thriveni, Girija Ramaswamy, Lakshmi Krishnamoorthy, Geetashree Mukherjee, P P Vijayalaxmi Deshmane, P P Bapsy.
Abstract
HER-2 is overexpressed in approximately 20-30% of invasive Breast Cancer. ECD of the HER-2 protein is frequently cleaved and released into the circulation, where it can be detected by ELISA in up to 45% of patients with metastatic breast cancer. The objective of our study was to compare the current methods for the detection of HER-2 protein. Tissue HER-2 levels were studied in 100 breast cancer patients by IHC and compared with serum HER-2 levels by ELISA. IHC frequency was 29%. Serum HER-2 ECD was positive in 42% of patients. A statistically significant correlation was observed. HER-2 detected by IHC correlates significantly with serum HER-2 levels detected by ELISA. Thus, ELISA is a reliable and economical tool to assess the HER-2 status in tumors, when breast tissue sample is not available.Entities:
Keywords: Breast cancer; ECD; ELISA; HER-2; IHC
Year: 2008 PMID： 23105784 PMCID： PMC3453142 DOI： 10.1007/s12291-008-0076-5
Source DB: PubMed Journal: Indian J Clin Biochem ISSN： 0970-1915