Literature DB >> 23105555

A sensitive and economical modified method for estimation of cerebrospinal fluid proteins.

Pushpa Durgawale1, Sudhir Kanase, Pramod S Shukla, Shubhangi Sontakke.   

Abstract

Cerebrospinal fluid protein estimation is a routine diagnostic tool for various diseases. The proposed modified bromocresol green (BCG) method is applicable to automated as well as manual measurements. Protein concentrations and absorbance at 600 nm are linearly related throughout a wide range of concentrations from 10 mg % to 2000 mg %. Ninety C.S.F. samples were analyzed and results obtained by both the modified BCG method and routine pyrogallol red method were compared. An excellent co-relation was observed (n=90, g=0.996, P<0.001).

Entities:  

Keywords:  C.S.F. protein; Modified Bromocresol green (BCG) method; Pyrogallol red method (Kit); SPSS 10.1 Statistical software

Year:  2005        PMID: 23105555      PMCID: PMC3453842          DOI: 10.1007/BF02867422

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN: 0970-1915


  7 in total

1.  Influence of reagent quality and reaction conditions on the determination of serum albumin by the bromcresol green dye-binding method.

Authors:  K Spencer; C P Price
Journal:  Ann Clin Biochem       Date:  1977-03       Impact factor: 2.057

2.  Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid.

Authors:  O MEULEMANS
Journal:  Clin Chim Acta       Date:  1960-09       Impact factor: 3.786

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  A study of the interaction of bromocresol green with isolated serum globulin fractions.

Authors:  D Webster
Journal:  Clin Chim Acta       Date:  1974-05-31       Impact factor: 3.786

6.  Some divergent results of protein standardization.

Authors:  J Booij
Journal:  Clin Chim Acta       Date:  1972-05       Impact factor: 3.786

7.  Urinary protein as measured with a pyrogallol red-molybdate complex, manually and in a Hitachi 726 automated analyzer.

Authors:  N Watanabe; S Kamei; A Ohkubo; M Yamanaka; S Ohsawa; K Makino; K Tokuda
Journal:  Clin Chem       Date:  1986-08       Impact factor: 8.327

  7 in total
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3.  Acute Methylglyoxal-Induced Damage in Blood-Brain Barrier and Hippocampal Tissue.

Authors:  Lílian Juliana Lissner; Krista Minéia Wartchow; Leticia Rodrigues; Larissa Daniele Bobermin; Ederson Borba; Vitor Gayger Dias; Fernanda Hansen; André Quincozes-Santos; Carlos-Alberto Gonçalves
Journal:  Neurotox Res       Date:  2022-09-03       Impact factor: 3.978

4.  Effects of intranasal guanosine administration on brain function in a rat model of ischemic stroke.

Authors:  Gabriel C Müller; Samanta O Loureiro; Letícia F Pettenuzzo; Roberto F Almeida; Evandro Y Ynumaru; Pedro A Guazzelli; Fabíola S Meyer; Mayara V Pasquetti; Marcelo Ganzella; Maria Elisa Calcagnotto; Diogo O Souza
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  4 in total

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