Literature DB >> 23105546

Production of recombinant enveloped structural proteins from the Chinese WSSV isolate.

Rajeev Kumar Jha1, Zi-Rong Xu.   

Abstract

The white spot syndrome virus (WSSV) is one of the deadly pathogens of penaeid shrimps and other crustaceans. The WSSV virion consists of an enveloped rod-shaped nucleocapsid enclosing a large circular double stranded DNA genome of 305 Kb with 181 open reading frames. The two major structural genes, VP19 and VP28 were amplified from the genomic DNA of Chinese isolate of WSSV and cloned in pUCm-T vector and sub cloned in pET-30a (+) vector. The expressions of genes inE. coli (BL21) were confirmed by SDS-PAGE analysis. The clones were sequenced, submitted to the gene bank and the Xiang Shan strain of WSSV were compared with the previous reported sequence of WSSV of various regions which revealed that VP19 and VP28 gene sequences had certain differences from the sequences of similar genes of the isolate already reported. The recombinant proteins expressed, purified and characterized.

Entities:  

Keywords:  Shrimp; VP19; VP28; White spot syndrome Virus

Year:  2005        PMID： 23105546      PMCID： PMC3453855          DOI： 10.1007/BF02867413

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  15 in total

1.  Identification of two major virion protein genes of white spot syndrome virus of shrimp.

Authors:  M C van Hulten; M Westenberg; S D Goodall; J M Vlak
Journal:  Virology       Date:  2000-01-20       Impact factor: 3.616

2.  Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication.

Authors:  Mariëlle C W van Hulten; Rob W Goldbach; Just M Vlak
Journal:  J Gen Virol       Date:  2000-10       Impact factor: 3.891

3.  Dot-blot nitrocellulose enzyme immunoassays for the detection of white-spot virus and yellow-head virus of penaeid shrimp.

Authors:  E C Nadala; P C Loh
Journal:  J Virol Methods       Date:  2000-02       Impact factor: 2.014

4.  Identification of genomic variations among geographic isolates of white spot syndrome virus using restriction analysis and Southern blot hybridization.

Authors:  Q Wang; L M Nunan; D V Lightner
Journal:  Dis Aquat Organ       Date:  2000-12-21       Impact factor: 1.802

5.  Development and application of monoclonal antibodies for the detection of white spot syndrome virus of penaeid shrimp.

Authors:  B T Poulos; C R Pantoja; D Bradley-Dunlop; J Aguilar; D V Lightner
Journal:  Dis Aquat Organ       Date:  2001-10-29       Impact factor: 1.802

6.  Genomic instability of prawn white spot bacilliform virus (WSBV) and its association to virus virulence.

Authors:  Yongsheng Lan; Wei Lu; Xun Xu
Journal:  Virus Res       Date:  2002-12       Impact factor: 3.303

7.  Highly conserved sequences of three major virion proteins of a Korean isolate of white spot syndrome virus (WSSV).

Authors:  Chang Hoon Moon; Jeong Wan Do; Seung Ju Cha; Won Joon Yoon; Seong Bum Kim; Myoung Seok Ko; Myoung Ae Park; Jin Woo Kim; Sang Kyu Sohn; Jong Hwa Lee; Jeong Woo Park
Journal:  Dis Aquat Organ       Date:  2003-01-22       Impact factor: 1.802

8.  Construction and characterization of a novel recombinant single-chain variable fragment antibody against White Spot Syndrome Virus from shrimp.

Authors:  Heping Dai; Hong Gao; Xinyan Zhao; Linfen Dai; Xiankong Zhang; Nan Xiao; Ruohong Zhao; Sean M Hemmingsen
Journal:  J Immunol Methods       Date:  2003-08       Impact factor: 2.303

9.  Proteomic analysis of shrimp white spot syndrome viral proteins and characterization of a novel envelope protein VP466.

Authors:  Canhua Huang; Xiaobo Zhang; Qingsong Lin; Xun Xu; Zhihong Hu; Choy-L Hew
Journal:  Mol Cell Proteomics       Date:  2002-03       Impact factor: 5.911

10.  Characterisation of shrimp haemocytes and plasma components by monoclonal antibodies.

Authors:  J Rodriguez; V Boulo; E Mialhe; E Bachere
Journal:  J Cell Sci       Date:  1995-03       Impact factor: 5.285
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.