Literature DB >> 23105498

Isolation and characterization of mitochondria from goat hearts.

Tester F Ashavaid1, Neena S Kumbhat.   

Abstract

Cardiac mitochondria provide energy for the contraction/relaxation cycle. The aim of our study was to isolate and characterize mitochondria from Caprine hearts under control and in-vitro induced ischemia. A decrease in activities of all the enzymes was observed in the ischemic models. Further characterization of proteins was done by SDS-PAGE and BN-PAGE. Lipids have been characterized by analyzing the phospholipids by HPTLC and fatty acids by GLC in both groups. Our results indicated that injury occurs early in the course of ischemia and progresses during ischemia. TBARS and carbonyl content have also been measured. The in-vitro effects of fatty acids have been studied on the enzymes and complexes of mitochondria.

Entities:  

Keywords:  Heart; ischemia; lipids; marker enzymes; respiratory chain complexes

Year:  2005        PMID： 23105498      PMCID： PMC3454148          DOI： 10.1007/BF02893046

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  44 in total

1.  A rapid spectrophotometric assay of mono-amine oxidase based on the rate of disappearance of kynuramine.

Authors:  H WEISSBACH; T E SMITH; J W DALY; B WITKOP; S UDENFRIEND
Journal:  J Biol Chem       Date:  1960-04       Impact factor: 5.157

2.  Mitochondrial function is differentially affected upon oxidative stress.

Authors:  S M Cardoso; C Pereira; R Oliveira
Journal:  Free Radic Biol Med       Date:  1999-01       Impact factor: 7.376

3.  Effect of ischemic preconditioning on mitochondrial oxidative phosphorylation and high energy phosphates in rat hearts.

Authors:  M Kobara; T Tatsumi; S Matoba; Y Yamahara; C Nakagawa; B Ohta; T Matsumoto; D Inoue; J Asayama; M Nakagawa
Journal:  J Mol Cell Cardiol       Date:  1996-02       Impact factor: 5.000

4.  Preparation and properties of succinate: ubiquinone oxidoreductase (complex II).

Authors:  Y Hatefi; D L Stiggall
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

5.  Arachidonic acid interaction with the mitochondrial electron transport chain promotes reactive oxygen species generation.

Authors:  T Cocco; M Di Paola; S Papa; M Lorusso
Journal:  Free Radic Biol Med       Date:  1999-07       Impact factor: 7.376

6.  Development of ischemia-induced damage in defined mitochondrial subpopulations.

Authors:  H M Piper; O Sezer; M Schleyer; P Schwartz; J F Hütter; P G Spieckermann
Journal:  J Mol Cell Cardiol       Date:  1985-09       Impact factor: 5.000

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Authors:  L A Sordahl; M L Stewart
Journal:  Circ Res       Date:  1980-12       Impact factor: 17.367

8.  Alterations in phospholipids in acute ischemic myocardium.

Authors:  T Yanagishita; N Konno; E Geshi; T Katagiri
Journal:  Jpn Circ J       Date:  1987-01

9.  Is monoamine oxidase activity in the outer mitochondrial membrane influenced by the mitochondrial respiratory state?

Authors:  A B Wojtczak; D Brdiczka; L Wojtczak
Journal:  Biochim Biophys Acta       Date:  1995-04-26

10.  An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.

Authors:  G L Sottocasa; B Kuylenstierna; L Ernster; A Bergstrand
Journal:  J Cell Biol       Date:  1967-02       Impact factor: 10.539
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  1 in total

1.  Caprine cardiac sarcoplasmic reticulum isolation and biochemical characterisation with emphasis on Ca(2+)-adenosine triphosphatase.

Authors:  Karen M D'Souza; Tester F Ashavaid
Journal:  Indian J Clin Biochem       Date:  2007-03
  1 in total

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