Literature DB >> 23105481

Study on construction of cDNA library of the treated changliver cell and quality analysis.

Lin Juntang1, Jogenananda Pramanik, Wang Congrui, Zhang Huiyong, Feng Huigen, Yang Baosheng, Li Yuchang, Xu Cunshuan.   

Abstract

The study aims to construct cDNA library of Changliver cell by SMART (switching mechanism at 5' end of RNA transcript) technique and analyze its quality. cDNA of Changliver cell was made with RT-PCR and LD-PCR (long-distance PCR), the cDNA library was constructed with SMART cDNA library construction kit. Through testing, the high quality cDNA library containing whole long cDNA of Changliver cell had been constructed. The titer of the amplified cDNA library was 4.5 × 10(10) pfu/ml and the average exogenous inserts of the recombinants is 1.5 kb. These results showed that the Changliver cell cDNA library had an excellent quality and lay foundation for screening whole long cDNA of related genes.

Keywords:  Changliver cell; LD-PCR; SMART; cDNA library; immunoscreening

Year:  2004        PMID： 23105481      PMCID： PMC3454184          DOI： 10.1007/BF02894282

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  10 in total

1.  Construction of a normalized cDNA library for the Trypanosoma cruzi genome project.

Authors:  T P Urményi; M F Bonaldo; M B Soares; E Rondinelli
Journal:  J Eukaryot Microbiol       Date:  1999 Sep-Oct       Impact factor: 3.346

Review 2.  Construction of full-length-enriched cDNA libraries. The oligo-capping method.

Authors:  Y Suzuki; S Sugano
Journal:  Methods Mol Biol       Date:  2001

3.  Study on construction of a cDNA library corresponding to an amino acid-specific tRNA and influence of the modified nucleotide upon nucleotide misincorporations in reverse transcription.

Authors:  J Matsugi; K Murao
Journal:  Biochim Biophys Acta       Date:  2001-10-31

4.  Construction and characterization of a vestibular-specific cDNA library using T7-based RNA amplification.

Authors:  Satoko Abe; Kumiko Koyama; Shin-ichi Usami; Yusuke Nakamura
Journal:  J Hum Genet       Date:  2003       Impact factor: 3.172

5.  Construction and characterization of protein libraries composed of secondary structure modules.

Authors:  Tomoaki Matsuura; Andreas Ernst; Andreas Plückthun
Journal:  Protein Sci       Date:  2002-11       Impact factor: 6.725

6.  Construction of Plasmodium falciparum lambda cDNA libraries.

Authors:  David A Fidock; Dharmendar Rathore; Thomas F McCutchan
Journal:  Methods Mol Med       Date:  2002

7.  Directional cDNA library construction assisted by the in vitro recombination reaction.

Authors:  O Ohara; G Temple
Journal:  Nucleic Acids Res       Date:  2001-02-15       Impact factor: 16.971

8.  Construction of cDNA libraries from microdissected benign and malignant thyroid tissue.

Authors:  Klaus Kaserer; Vladimir Knezevic; Bettina Pichlhöfer; Christian Scheuba; Christian Passler; Jennifer Worth; Bruno Niederle; David Krizman
Journal:  Lab Invest       Date:  2002-12       Impact factor: 5.662

9.  Construction of a DNA library from chromosome 4 of rice (Oryza sativa) by microdissection.

Authors:  Y W Mao; S Y Liang; W Q Song; X L Li; R Y Chen
Journal:  Cell Res       Date:  1998-12       Impact factor: 25.617

10.  Simplified, rapid method for cloning of virus-binding polypeptides (putative receptors) via the far-western screening of a cDNA expression library using purified virus particles.

Authors:  R B Medeiros; L Rasochova; T L German
Journal:  J Virol Methods       Date:  2000-05       Impact factor: 2.014
  10 in total

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