Literature DB >> 23105346

HPLC method for amino acids profile in biological fluids and inborn metabolic disorders of aminoacidopathies.

S V Suresh Babu1, M M Shareef, A Pavan Kumar Shetty, K Taranath Shetty.   

Abstract

Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino acids by modified method based on dinitrophenyl and HPLC profile involving pre-column derivatization with o-pthalaldehyde (OPA) derivatization, respectively. The method, based on the alkylation of-SH groups prior to OPA derivatization of amino acids followed by reverse phase high performance liquid chromatography, provide a comprehensive profile of more than twenty amino acids (including-SH group containing) in a single run lasting about 45 minutes. The present study, apart from establishing the normal profile of amino acids in plasma of Indian sub population, also presents HPLC profile for some of the rare amino acidopathies.

Entities:  

Keywords:  Inborn metabolic disorders; O-pthaladehyde; amino acidopathies; amino acids profile; reverse phase HPLC

Year:  2002        PMID： 23105346      PMCID： PMC3454125          DOI： 10.1007/BF02867967

Source DB:  PubMed          Journal:  Indian J Clin Biochem        ISSN： 0970-1915


  18 in total

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7.  Mechanism of nitrogen metabolism-related parameters and enzyme activities in the pathophysiology of autism.

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Authors:  Miriam van Dijk; Francina J Dijk; Annelies Bunschoten; Dorien A M van Dartel; Klaske van Norren; Stephane Walrand; Marion Jourdan; Sjors Verlaan; Yvette Luiking
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  8 in total

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