| Literature DB >> 23105319 |
S Banerjee1, E R Nair, S Kumar, M V Reddy, B C Harinath.
Abstract
Analysis of tubercular antibody, circulating free and immune complexed antigen (CIC-Ag) was done in confimed pulmonary tuberculosis sera by ELISA, using ES-31 antigen and affinity purified anti ES-31 antibody. Twenty three of 25 (92%) tuberculosis sera were positive for IgG antibody to ES-31 antigen. Using anti ES-31 antibody, free tubercular antigen could be detected in 20 of 25 (80%) cases whereas circulating immune complexed antigen (CIC-Ag) in 18 of 25 (72%) cases by sandwich ELISA. Of the two sera showing absence of antibody, one showed presence of free and CIC-Ag whereas the other showed the presence of free antigen. Thus antigen assay may be used as an adjunct tool for confirmation of pulmonary tuberculosis.Entities:
Keywords: Antibody; Antigen; ELISA; ES-31 Antigen; Immunodiagnosis; Pulmonary tuberculosis
Year: 2001 PMID： 23105319 PMCID： PMC3453637 DOI： 10.1007/BF02864862
Source DB: PubMed Journal: Indian J Clin Biochem ISSN： 0970-1915