AIMS: Capillary zone electrophoresis (CE) at alkaline pH is increasingly used in haemoglobinopathy diagnosis. We report our evaluation of automated CE, using the Capillarys 2 Flex Piercing instrument, as a routine diagnostic method for the detection of variant haemoglobins and the diagnosis of β thalassaemia. METHODS: A Capillarys 2 Flex Piercing instrument with Phoresis software was evaluated in our laboratory over a 6-week period, comparisons being made with high performance liquid chromatography (HPLC) and, for haemoglobin A(2) quantification, with microcolumn chromatography. RESULTS: The instrument was easy to use and was suitable for the quantification of haemoglobin A(2). Quantification of A(2) was precise and the percentage was stable with ageing of the blood specimen. Results differ among HPLC, CE and microcolumn chromatography and use of an instrument-specific, method-specific reference range is therefore recommended until such time as there is standardisation between methods and manufacturers. Common variant haemoglobins were provisionally identified without difficulty. There are some uncommon variant haemoglobins that are detected by HPLC but not by capillary electrophoresis, but the reverse also occurs. CONCLUSIONS: Capillary electrophoresis using a Capillarys 2 Flex Piercing instrument is suitable for haemoglobinopathy diagnosis.
AIMS: Capillary zone electrophoresis (CE) at alkaline pH is increasingly used in haemoglobinopathy diagnosis. We report our evaluation of automated CE, using the Capillarys 2 Flex Piercing instrument, as a routine diagnostic method for the detection of variant haemoglobins and the diagnosis of β thalassaemia. METHODS: A Capillarys 2 Flex Piercing instrument with Phoresis software was evaluated in our laboratory over a 6-week period, comparisons being made with high performance liquid chromatography (HPLC) and, for haemoglobin A(2) quantification, with microcolumn chromatography. RESULTS: The instrument was easy to use and was suitable for the quantification of haemoglobin A(2). Quantification of A(2) was precise and the percentage was stable with ageing of the blood specimen. Results differ among HPLC, CE and microcolumn chromatography and use of an instrument-specific, method-specific reference range is therefore recommended until such time as there is standardisation between methods and manufacturers. Common variant haemoglobins were provisionally identified without difficulty. There are some uncommon variant haemoglobins that are detected by HPLC but not by capillary electrophoresis, but the reverse also occurs. CONCLUSIONS: Capillary electrophoresis using a Capillarys 2 Flex Piercing instrument is suitable for haemoglobinopathy diagnosis.
Authors: Muhammad Noman Hasan; Arwa Fraiwan; Ran An; Yunus Alapan; Ryan Ung; Asya Akkus; Julia Z Xu; Amy J Rezac; Nicholas J Kocmich; Melissa S Creary; Tolulope Oginni; Grace Mfon Olanipekun; Fatimah Hassan-Hanga; Binta W Jibir; Safiya Gambo; Anil K Verma; Praveen K Bharti; Suchada Riolueang; Takdanai Ngimhung; Thidarat Suksangpleng; Priyaleela Thota; Greg Werner; Rajasubramaniam Shanmugam; Aparup Das; Vip Viprakasit; Connie M Piccone; Jane A Little; Stephen K Obaro; Umut A Gurkan Journal: Analyst Date: 2020-03-03 Impact factor: 4.616
Authors: Hee Won Chueh; Sang Mee Hwang; Ye Jee Shim; Jae Min Lee; Hee Sue Park; Joon Hee Lee; Youngwon Nam; Namhee Kim; Hye Lim Jung; Hyoung Soo Choi Journal: Blood Res Date: 2022-05-20