Literature DB >> 2310383

Quantitative analysis of human immunodeficiency virus type-1 DNA in asymptomatic carriers using the polymerase chain reaction.

S Oka1, K Urayama, Y Hirabayashi, K Ohnishi, H Goto, K Mitamura, S Kimura, K Shimada.   

Abstract

A method for detecting human immunodeficiency virus type 1 (HIV-1) provirus DNA in lymphocytes with improved sensitivity and reproducibility was developed using the polymerase chain reaction (PCR). Amplified HIV-1 DNA was hybridized with a 32P-labeled probe and quantitated with a beta-scanner after electrophoresis. A linear relationship was obtained between the common logarithms of the counts detected and the number of HIV-1 DNA copies applied to the PCR. Detectability was from 3 copies/10(5) lymphocytes, and linearity was maintained from 10 to 10(3) copies. HIV-1 DNA was detected in all 9 asymptomatic carriers tested (18 to 2,857 copies/10(5) CD4+ T lymphocytes). The viral burden was inversely related to the CD4+ lymphocyte count, suggesting that quantitation of provirus levels may serve as a predictor of progress in early HIV infection.

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Year:  1990        PMID： 2310383     DOI： 10.1016/0006-291x(90)91721-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  8 in total

1.  Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.

Authors:  A J Whetsell; J B Drew; G Milman; R Hoff; E A Dragon; K Adler; J Hui; P Otto; P Gupta; H Farzadegan
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

Review 2.  Clinical use of quantitative molecular methods in studying human immunodeficiency virus type 1 infection.

Authors:  M Clementi; S Menzo; P Bagnarelli; A Valenza; S Paolucci; R Sampaolesi; A Manzin; P E Varaldo
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

Review 3.  Quantitative molecular methods in virology.

Authors:  M Clementi; S Menzo; A Manzin; P Bagnarelli
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

4.  Quantitative molecular monitoring of human immunodeficiency virus type 1 activity during therapy with specific antiretroviral compounds.

Authors:  P Bagnarelli; S Menzo; A Valenza; S Paolucci; S Petroni; G Scalise; R Sampaolesi; A Manzin; P E Varaldo; M Clementi
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

5.  Standardized nested polymerase chain reaction-based assay for detection of human immunodeficiency virus type 1 DNA in whole blood lysates.

Authors:  S Sauvaigo; V Barlet; N Guettari; P Innocenti; F Parmentier; C Bastard; J M Seigneurin; J C Chermann; R Teoule; J Marchand
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

6.  Quantification of human immunodeficiency virus in plasma by RNA PCR, viral culture, and p24 antigen detection.

Authors:  I Van Kerckhoven; K Fransen; M Peeters; H De Beenhouwer; P Piot; G van der Groen
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

7.  Non-radioactive PCR method for quantification of HIV-infected peripheral blood mononuclear cells in HIV-positive subjects.

Authors:  M van der Feltz; H S Nottet; M R Visser; J C Borleffs; J Verhoef
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-12       Impact factor: 3.267

8.  Dynamics of molecular parameters of human immunodeficiency virus type 1 activity in vivo.

Authors:  P Bagnarelli; A Valenza; S Menzo; A Manzin; G Scalise; P E Varaldo; M Clementi
Journal:  J Virol       Date:  1994-04       Impact factor: 5.103
  8 in total

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