PURPOSE OF THE STUDY: Study was focused to effectively suppress the agglutinability of human A and B type erythrocyte by concerted chemical oxidation approach. Consequently some major bio-physiological consequences of human erythrocytes were analyzed. MATERIALS AND METHODS: Concerted chemical oxidation of human A and B type erythrocyte were done by potassium permanganate (KMnO(4)) in presence of ethylene diamine tetra-acetic acid (EDTA). All the chemical reactions were carried out at 37°C temperature and pH 7.4 in phosphate buffer. Finally, suppression of agglutinability were determined by using anti-A and anti-B antisera. RESULTS: A marked suppression in agglutinability was observed after chemical treatment on human A and B type erythrocytes in vitro. Effective suppression of agglutinability was observed in 0.57 mM concentration of KMnO(4) in presence of 0.9 mM EDTA at optimum condition. CONCLUSIONS: Results showed that KMnO(4)-EDTA concerted action is feasible. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.
PURPOSE OF THE STUDY: Study was focused to effectively suppress the agglutinability of human A and B type erythrocyte by concerted chemical oxidation approach. Consequently some major bio-physiological consequences of human erythrocytes were analyzed. MATERIALS AND METHODS: Concerted chemical oxidation of human A and B type erythrocyte were done by potassium permanganate (KMnO(4)) in presence of ethylene diamine tetra-acetic acid (EDTA). All the chemical reactions were carried out at 37°C temperature and pH 7.4 in phosphate buffer. Finally, suppression of agglutinability were determined by using anti-A and anti-B antisera. RESULTS: A marked suppression in agglutinability was observed after chemical treatment on human A and B type erythrocytes in vitro. Effective suppression of agglutinability was observed in 0.57 mM concentration of KMnO(4) in presence of 0.9 mM EDTA at optimum condition. CONCLUSIONS: Results showed that KMnO(4)-EDTA concerted action is feasible. The future work is directed towards the universal human erythrocytes preparation from A and B type human erythrocytes.
Entities:
Keywords:
Agglutination; Antibodies; Concerted action; Erythrocyte; Transfusion medicine
Authors: Smita Narayanan; Lamar Galloway; Akihisa Nonoyama; German F Leparc; Luis H Garcia-Rubio; Robert L Potter Journal: Transfusion Date: 2002-05 Impact factor: 3.157