Literature DB >> 23100780

Cloning, expression and immunogenicity of ferric enterobactin binding protein Fep B from Escherichia coli O157:H7.

Majid Alipour1, Seyed Latif Mousavi Gargari, Iraj Rasooli.   

Abstract

The gene coding for ferric enterobactin binding protein from E. coli O157:H7 was amplifi ed. This gene was cloned and expressed as C-terminal His (6)-tagged protein. The SDS-PAGE analysis of the total protein revealed only two distinct bands, with molecular masses of 31kDa and 34kDa. The Ni-NTA chromatography purifi ed FepB and the osmotically shocked periplasmic fraction of IPTG induced cells showed only a single band of 31 kDa. Polyclonal mouse antibody was raised against the recombinant protein during 4 weeks after immunization. Western blot analysis of the recombinant FepB with mouse antiserum revealeda single band of 31 kDa. Identification and purification of FepB helped reveal its appropriate molecular mass. Polyclonal antibody raised against the recombinant protein reacted with bacterial FepB. The recombinant protein FepB could have a protective effect against E. coli O157:H7 and might be useful as an effective vaccine.

Entities:  

Keywords:  E. coli; FepB; Ferric enterobactin; Periplasmic binding protein

Year:  2009        PMID: 23100780      PMCID: PMC3450013          DOI: 10.1007/s12088-009-0044-7

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN: 0046-8991            Impact factor:   2.461


  13 in total

1.  Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli.

Authors:  C M Shea; M A McIntosh
Journal:  Mol Microbiol       Date:  1991-06       Impact factor: 3.501

2.  Active transport by customized beta-barrels.

Authors:  K Postle
Journal:  Nat Struct Biol       Date:  1999-01

3.  Identification of an AU-rich translational enhancer within the Escherichia coli fepB leader RNA.

Authors:  India G Hook-Barnard; Timothy J Brickman; Mark A McIntosh
Journal:  J Bacteriol       Date:  2007-03-30       Impact factor: 3.490

4.  Binding of ferric enterobactin by the Escherichia coli periplasmic protein FepB.

Authors:  C Sprencel; Z Cao; Z Qi; D C Scott; M A Montague; N Ivanoff; J Xu; K M Raymond; S M Newton; P E Klebba
Journal:  J Bacteriol       Date:  2000-10       Impact factor: 3.490

5.  TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli.

Authors:  Corinna Bleuel; Cornelia Grosse; Nadine Taudte; Judith Scherer; Dirk Wesenberg; Gerd J Krauss; Dietrich H Nies; Gregor Grass
Journal:  J Bacteriol       Date:  2005-10       Impact factor: 3.490

Review 6.  Feo--transport of ferrous iron into bacteria.

Authors:  Michaël L Cartron; Sarah Maddocks; Paul Gillingham; C Jeremy Craven; Simon C Andrews
Journal:  Biometals       Date:  2006-04       Impact factor: 2.949

Review 7.  Bacterial iron sources: from siderophores to hemophores.

Authors:  Cécile Wandersman; Philippe Delepelaire
Journal:  Annu Rev Microbiol       Date:  2004       Impact factor: 15.500

Review 8.  How pathogenic bacteria evade mammalian sabotage in the battle for iron.

Authors:  Michael A Fischbach; Hening Lin; David R Liu; Christopher T Walsh
Journal:  Nat Chem Biol       Date:  2006-03       Impact factor: 15.040

9.  Iron-source preference of Staphylococcus aureus infections.

Authors:  Eric P Skaar; Munir Humayun; Taeok Bae; Kristin L DeBord; Olaf Schneewind
Journal:  Science       Date:  2004-09-10       Impact factor: 47.728

Review 10.  Iron(III) hydroxamate transport across the cytoplasmic membrane of Escherichia coli.

Authors:  W Köster
Journal:  Biol Met       Date:  1991
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  1 in total

1.  A Serendipitous Mutation Reveals the Severe Virulence Defect of a Klebsiella pneumoniae fepB Mutant.

Authors:  Michelle Palacios; Christopher A Broberg; Kimberly A Walker; Virginia L Miller
Journal:  mSphere       Date:  2017-08-23       Impact factor: 4.389

  1 in total

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