| Literature DB >> 23100478 |
Alvina Clara Felix1, Camila Malta Romano, Cristiane de Campos Centrone, Célia Lima Rodrigues, Lucy Villas-Boas, Evaldo Stanislau Araújo, Andréia Manso de Matos, Karina Inácio Carvalho, Celina Maria Turchi Martelli, Esper Georges Kallas, Claúdio Sérgio Pannuti, José Eduardo Levi.
Abstract
In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary infection by dengue 2 virus. Sequencing of NS1 positive and negative isolates did not reveal any mutation that could justify the diagnostic failure. Use of existing NS1 tests in the Brazilian population may present a low negative predictive value, and they should be used with caution, preferentially after performing a validation with samples freshly obtained during the ongoing epidemic.Entities:
Mesh：
Substances：
Year: 2012 PMID： 23100478 PMCID： PMC3535870 DOI： 10.1128/CVI.00535-12
Source DB: PubMed Journal: Clin Vaccine Immunol ISSN： 1556-679X