Literature DB >> 23099912

Immobilized metal ion affinity chromatography: a review on its applications.

Randy Chi Fai Cheung1, Jack Ho Wong, Tzi Bun Ng.   

Abstract

After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has evolved into a popular protein purification technique. This review starts with a discussion of its mechanism and advantages. It continues with its applications which include the purification of histidine-tagged proteins, natural metal-binding proteins, and antibodies. IMAC used in conjunction with mass spectroscopy for phosphoprotein fractionation and proteomics is also covered. Finally, this review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.

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Year:  2012        PMID： 23099912     DOI： 10.1007/s00253-012-4507-0

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  30 in total

Review 1.  Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.

Authors:  Christine F Markwalter; Andrew G Kantor; Carson P Moore; Kelly A Richardson; David W Wright
Journal:  Chem Rev       Date:  2018-12-04       Impact factor: 60.622

Review 2.  Affinity chromatography: A review of trends and developments over the past 50 years.

Authors:  Elliott L Rodriguez; Saumen Poddar; Sazia Iftekhar; Kyungah Suh; Ashley G Woolfork; Susan Ovbude; Allegra Pekarek; Morgan Walters; Shae Lott; David S Hage
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2020-08-14       Impact factor: 3.205

3.  Size of Protein is a Major Factor that Affects Retention on Preparative IMAC Columns.

Authors:  Carmel N Tovar; Odutayo O Odunuga
Journal:  Protein J       Date:  2019-02       Impact factor: 2.371

4.  Tyrosine-Based Cross-Linking of Peptide Antigens to Generate Nanoclusters with Enhanced Immunogenicity: Demonstration Using the Conserved M2e Peptide of Influenza A.

Authors:  Logan R Wilks; Gaurav Joshi; Megan R Grisham; Harvinder Singh Gill
Journal:  ACS Infect Dis       Date:  2021-08-25       Impact factor: 5.578

5.  Process for production and purification of Lethal Toxin Neutralizing Factor (LTNF) from E. coli and its economic analysis.

Authors:  Vishwanath Hebbi; P Kathiresan; Devendra Kumar; Claire Komives; Anurag S Rathore
Journal:  J Chem Technol Biotechnol       Date:  2017-12-05       Impact factor: 3.174

6.  Assessing histidine tags for recruiting deoxyribozymes to catalyze peptide and protein modification reactions.

Authors:  Chih-Chi Chu; Scott K Silverman
Journal:  Org Biomol Chem       Date:  2016-05-18       Impact factor: 3.876

7.  T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis.

Authors:  Ana Filipa Sequeira; Catarina I P D Guerreiro; Renaud Vincentelli; Carlos M G A Fontes
Journal:  Mol Biotechnol       Date:  2016-09       Impact factor: 2.695

8.  Investigating direct interaction between Escherichia coli topoisomerase I and RecA.

Authors:  Srikanth Banda; Purushottam Babu Tiwari; Yesim Darici; Yuk-Ching Tse-Dinh
Journal:  Gene       Date:  2016-03-19       Impact factor: 3.688

9.  PET imaging of 64Cu-DOTA-scFv-anti-PSMA lipid nanoparticles (LNPs): Enhanced tumor targeting over anti-PSMA scFv or untargeted LNPs.

Authors:  Patty Wong; Lin Li; Junie Chea; Melissa K Delgado; Desiree Crow; Erasmus Poku; Barbara Szpikowska; Nicole Bowles; Divya Channappa; David Colcher; Jeffrey Y C Wong; John E Shively; Paul J Yazaki
Journal:  Nucl Med Biol       Date:  2017-01-17       Impact factor: 2.408

10.  In situ magnetic separation of antibody fragments from Escherichia coli in complex media.

Authors:  Martin Cerff; Alexander Scholz; Matthias Franzreb; Iris L Batalha; Ana Cecilia A Roque; Clemens Posten
Journal:  BMC Biotechnol       Date:  2013-05-20       Impact factor: 2.563
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