OBJECTIVE: This study investigates the effects of noradrenaline (NA) on cytoskeletal protein expression of vascular smooth muscle cells (VSMCs). METHODS: VSMCs were isolated from rat aortic tissue and cultured. The cultured VSMCs were divided into 4 experimental groups: (1) control group, (2) NA treatment group, (3) starvation group, and (4) NA treatment+starvation group. The expression of cytoskeletal protein (smooth muscle α-actin, β-tubulin and desmin) was evaluated by (i) Coomassie blue staining, (ii) immunofluorescent staining, and (iii) RT-PCR and Western Blot. RESULTS: NA treatment significantly downregulated the expression of SM α-actin, β-tubulin and desmin (P<0.05). The serum starvation did not affect the expression of cytoskeletal protein (SM α-actin, β-tubulin and desmin), but when the cells were treated with the combination of NA and serum starvation, the expression of SM α-actin, β-tubulin and desmin were down-regulated than those of the serum starvation group (P<0.05). CONCLUSION: These results suggested that NA might play a key role in regulating the cytoskeletal protein expression of VSMCs.
OBJECTIVE: This study investigates the effects of noradrenaline (NA) on cytoskeletal protein expression of vascular smooth muscle cells (VSMCs). METHODS: VSMCs were isolated from rat aortic tissue and cultured. The cultured VSMCs were divided into 4 experimental groups: (1) control group, (2) NA treatment group, (3) starvation group, and (4) NA treatment+starvation group. The expression of cytoskeletal protein (smooth muscle α-actin, β-tubulin and desmin) was evaluated by (i) Coomassie blue staining, (ii) immunofluorescent staining, and (iii) RT-PCR and Western Blot. RESULTS: NA treatment significantly downregulated the expression of SM α-actin, β-tubulin and desmin (P<0.05). The serum starvation did not affect the expression of cytoskeletal protein (SM α-actin, β-tubulin and desmin), but when the cells were treated with the combination of NA and serum starvation, the expression of SM α-actin, β-tubulin and desmin were down-regulated than those of the serum starvation group (P<0.05). CONCLUSION: These results suggested that NA might play a key role in regulating the cytoskeletal protein expression of VSMCs.
Authors: Daniely Ferreira de Queiroz; Tony Rogério de Lima Dadamos; Sergio Antonio Spinola Machado; Marco Antonio Utrera Martines Journal: Sensors (Basel) Date: 2018-04-16 Impact factor: 3.576