Literature DB >> 23089313

Inducing local DNA damage by visible light to study chromatin repair.

Kamil J Solarczyk1, Mirosław Zarębski, Jurek W Dobrucki.   

Abstract

Dynamics of DNA repair and recruitment of repair factors to damaged DNA can be studied by live cell microscopy. DNA damage is usually inflicted by a laser beam illuminating a DNA-interacting photosensitizer in a small area of the nucleus. We demonstrate that a focused beam of visible low intensity light alone can inflict local DNA damage and permit studies of DNA repair, thus avoiding potential artifacts caused by exogenous photosensitizers.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID： 23089313     DOI： 10.1016/j.dnarep.2012.09.008

Source DB:  PubMed          Journal:  DNA Repair (Amst)        ISSN： 1568-7856


  11 in total

Review 1.  Micro-irradiation tools to visualize base excision repair and single-strand break repair.

Authors:  Natalie R Gassman; Samuel H Wilson
Journal:  DNA Repair (Amst)       Date:  2015-05-05

2.  Dynamics of the HP1β-PCNA-containing complexes in DNA replication and repair.

Authors:  Dominika O Trembecka-Lucas; Aleksander T Szczurek; Jurek W Dobrucki
Journal:  Nucleus       Date:  2013-01-01       Impact factor: 4.197

3.  PML-like subnuclear bodies, containing XRCC1, juxtaposed to DNA replication-based single-strand breaks.

Authors:  Magdalena M Kordon; Aleksander Szczurek; Krzysztof Berniak; Oskar Szelest; Kamil Solarczyk; Magdalena Tworzydło; Sebastian Wachsmann-Hogiu; Anne Vaahtokari; Christoph Cremer; Thoru Pederson; Jurek W Dobrucki
Journal:  FASEB J       Date:  2018-09-27       Impact factor: 5.834

4.  Highlighting the DNA damage response with ultrashort laser pulses in the near infrared and kinetic modeling.

Authors:  Elisa Ferrando-May; Martin Tomas; Philipp Blumhardt; Martin Stöckl; Matthias Fuchs; Alfred Leitenstorfer
Journal:  Front Genet       Date:  2013-07-16       Impact factor: 4.599

5.  Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos.

Authors:  Rosemary H C Wilson; Dawn Coverley
Journal:  Sci Rep       Date:  2017-07-25       Impact factor: 4.379

6.  Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells.

Authors:  Nathaniel W Holton; Joel F Andrews; Natalie R Gassman
Journal:  J Vis Exp       Date:  2017-09-05       Impact factor: 1.355

7.  The study of protein recruitment to laser-induced DNA lesions can be distorted by photoconversion of the DNA binding dye Hoechst.

Authors:  Verena Hurst; Susan M Gasser
Journal:  F1000Res       Date:  2019-01-25

8.  Systematic analysis of DNA damage induction and DNA repair pathway activation by continuous wave visible light laser micro-irradiation.

Authors:  Britta Muster; Alexander Rapp; M Cristina Cardoso
Journal:  AIMS Genet       Date:  2017-02-21

9.  Quantifying Multistate Cytoplasmic Molecular Diffusion in Bacterial Cells via Inverse Transform of Confined Displacement Distribution.

Authors:  Tai-Yen Chen; Won Jung; Ace George Santiago; Feng Yang; Łukasz Krzemiński; Peng Chen
Journal:  J Phys Chem B       Date:  2015-11-02       Impact factor: 2.991

10.  STRIDE-a fluorescence method for direct, specific in situ detection of individual single- or double-strand DNA breaks in fixed cells.

Authors:  Magdalena M Kordon; Mirosław Zarębski; Kamil Solarczyk; Hanhui Ma; Thoru Pederson; Jurek W Dobrucki
Journal:  Nucleic Acids Res       Date:  2020-02-20       Impact factor: 16.971
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