Da-wei Li1, Liang Zhou, Bin Jin, Jin Xie, Pin Dong. 1. Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye and ENT Hospital of Fudan University, Shanghai, China.
Abstract
OBJECTIVE: The aim was to evaluate hypoxia-inducible factor-1α (HIF-1α) and survivin expression in laryngeal squamous cell carcinoma (LSCC) tissues and cell lines and to investigate whether HIF-1α has an effect on the regulation of survivin gene expression in LSCC cells under hypoxia. STUDY DESIGN: Prospective, observational. SETTING: Shanghai Jiaotong University Affiliated First People's Hospital. SUBJECTS AND METHODS: The expression of HIF-1α and survivin protein in human LSCC tissues was analyzed by immunohistochemistry. HIF-1α and survivin gene expression levels in Hep-2 cells were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot under normoxic or hypoxic conditions. In hypoxic cells, HIF-1α expression was inhibited by RNA interference. RESULTS: HIF-1α and survivin were both highly expressed in LSCC tissues and significantly related to the clinical stage and lymph node metastasis (P < .05). Meanwhile, a positive correlation existed between HIF-1α and survivin expression (r = 0.456, P < .01). In LSCC cells, HIF-1α and survivin expression were obviously upregulated in response to hypoxia (P < .05). The downregulation of HIF-1α expression dramatically decreased survivin gene expression in hypoxic cells (P < .05). CONCLUSION: HIF-1α could be considered as an important regulator for the upregulation of survivin gene expression induced by hypoxia in LSCC cells, and both proteins could be regarded as 2 key predictors of malignant progression and metastasis of LSCC.
OBJECTIVE: The aim was to evaluate hypoxia-inducible factor-1α (HIF-1α) and survivin expression in laryngeal squamous cell carcinoma (LSCC) tissues and cell lines and to investigate whether HIF-1α has an effect on the regulation of survivin gene expression in LSCC cells under hypoxia. STUDY DESIGN: Prospective, observational. SETTING: Shanghai Jiaotong University Affiliated First People's Hospital. SUBJECTS AND METHODS: The expression of HIF-1α and survivin protein in human LSCC tissues was analyzed by immunohistochemistry. HIF-1α and survivin gene expression levels in Hep-2 cells were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot under normoxic or hypoxic conditions. In hypoxic cells, HIF-1α expression was inhibited by RNA interference. RESULTS: HIF-1α and survivin were both highly expressed in LSCC tissues and significantly related to the clinical stage and lymph node metastasis (P < .05). Meanwhile, a positive correlation existed between HIF-1α and survivin expression (r = 0.456, P < .01). In LSCC cells, HIF-1α and survivin expression were obviously upregulated in response to hypoxia (P < .05). The downregulation of HIF-1α expression dramatically decreased survivin gene expression in hypoxic cells (P < .05). CONCLUSION: HIF-1α could be considered as an important regulator for the upregulation of survivin gene expression induced by hypoxia in LSCC cells, and both proteins could be regarded as 2 key predictors of malignant progression and metastasis of LSCC.