INTRODUCTION: Podocytes can respond to various injuries, including mechanical stress secondary to diabetic nephropathy (DN), which may cause deleterious adhesive effects on podocytes. Integrin α3β1 is the major podocyte adhesion molecule. In this study, we aim to investigate α3β1 expression and identify differentially expressed microRNAs in podocytes under mechanical stress compared with normal cells and podocytes under mechanical stress treated with spironolactone, respectively. MATERIALS AND METHODS: Serum and glucocorticoid induced kinase 1 (SGK1), mineralocorticoid receptor (MR) and integrin α3β1 were detected by Western blotting. The miRNA analyses were performed by TaqMan MicroRNA Array v2.0. Genes Itga3 and Itgb1 were analyzed for miRNA binding sites within 3'UTRs using TargetScan and PicTar. RESULTS: Protein SGK1 and MR expression were significantly increased under mechanical stress and decreased after spironolactone treatment. Podocyte α3 and β1 expression were significantly decreased under mechanical stress and increased after spironolactone treatment. MiR-124, miR-190, miR-217 and miR-188 were the overlapped miRNAs that were upregulated under mechanical stress and downregulated after spironolactone treatment. MiR-124 was found to be a predicted miRNA target site in both Itga3 and Itgb1 3'UTRs. CONCLUSION: These results provide a novel idea that miR-124 might play an important role in podocytic adhesion damage under mechanical stress.
INTRODUCTION: Podocytes can respond to various injuries, including mechanical stress secondary to diabetic nephropathy (DN), which may cause deleterious adhesive effects on podocytes. Integrin α3β1 is the major podocyte adhesion molecule. In this study, we aim to investigate α3β1 expression and identify differentially expressed microRNAs in podocytes under mechanical stress compared with normal cells and podocytes under mechanical stress treated with spironolactone, respectively. MATERIALS AND METHODS:Serum and glucocorticoid induced kinase 1 (SGK1), mineralocorticoid receptor (MR) and integrin α3β1 were detected by Western blotting. The miRNA analyses were performed by TaqMan MicroRNA Array v2.0. Genes Itga3 and Itgb1 were analyzed for miRNA binding sites within 3'UTRs using TargetScan and PicTar. RESULTS: Protein SGK1 and MR expression were significantly increased under mechanical stress and decreased after spironolactone treatment. Podocyte α3 and β1 expression were significantly decreased under mechanical stress and increased after spironolactone treatment. MiR-124, miR-190, miR-217 and miR-188 were the overlapped miRNAs that were upregulated under mechanical stress and downregulated after spironolactone treatment. MiR-124 was found to be a predicted miRNA target site in both Itga3 and Itgb1 3'UTRs. CONCLUSION: These results provide a novel idea that miR-124 might play an important role in podocytic adhesion damage under mechanical stress.
Authors: Janina Müller-Deile; Jan Dannenberg; Patricia Schroder; Meei-Hua Lin; Jeffrey H Miner; Rongjun Chen; Jan-Hinrich Bräsen; Thomas Thum; Jenny Nyström; Lynne Beverly Staggs; Hermann Haller; Jan Fiedler; Johan M Lorenzen; Mario Schiffer Journal: Kidney Int Date: 2017-05-03 Impact factor: 10.612
Authors: Amanda Tivnan; Jack Zhao; Terrance G Johns; Bryan W Day; Brett W Stringer; Andrew W Boyd; Sarita Tiwari; Keith M Giles; Charlie Teo; Kerrie L McDonald Journal: Tumour Biol Date: 2013-09-26
Authors: Anke Ruedel; Peter Dietrich; Thomas Schubert; Simone Hofmeister; Claus Hellerbrand; Anja Katrin Bosserhoff Journal: Int J Clin Exp Pathol Date: 2015-06-01