Literature DB >> 23087036

Identification of a residue affecting fatty alcohol selectivity in wax ester synthase.

Brett M Barney1, Rachel L Mann, Janet M Ohlert.   

Abstract

The terminal enzyme in the bacterial wax ester biosynthetic pathway is the bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT), which utilizes a fatty alcohol and a fatty acyl-coenzyme A (CoA) to synthesize the corresponding wax ester. In this report, we identify a specific residue in WS/DGAT enzymes obtained from Marinobacter aquaeolei VT8 and Acinetobacter baylyi that alters fatty alcohol selectivity and kinetic parameters when modified to alternative residues.

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Year:  2012        PMID： 23087036      PMCID： PMC3536089          DOI： 10.1128/AEM.02523-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  20 in total

1.  Isolation of mutants of Acinetobacter calcoaceticus deficient in wax ester synthesis and complementation of one mutation with a gene encoding a fatty acyl coenzyme A reductase.

Authors:  S Reiser; C Somerville
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

2.  Microdiesel: Escherichia coli engineered for fuel production.

Authors:  Rainer Kalscheuer; Torsten Stölting; Alexander Steinbüchel
Journal:  Microbiology       Date:  2006-09       Impact factor: 2.777

3.  Crystal structures of murine carnitine acetyltransferase in ternary complexes with its substrates.

Authors:  Yu-Shan Hsiao; Gerwald Jogl; Liang Tong
Journal:  J Biol Chem       Date:  2006-07-26       Impact factor: 5.157

4.  Differences in substrate specificities of five bacterial wax ester synthases.

Authors:  Brett M Barney; Bradley D Wahlen; EmmaLee Garner; Jiashi Wei; Lance C Seefeldt
Journal:  Appl Environ Microbiol       Date:  2012-06-08       Impact factor: 4.792

5.  The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase.

Authors:  Tim Stöveken; Rainer Kalscheuer; Ursula Malkus; Rudolf Reichelt; Alexander Steinbüchel
Journal:  J Bacteriol       Date:  2005-02       Impact factor: 3.490

6.  Biosynthesis of isoprenoid wax ester in Marinobacter hydrocarbonoclasticus DSM 8798: identification and characterization of isoprenoid coenzyme A synthetase and wax ester synthases.

Authors:  Erik Holtzapple; Claudia Schmidt-Dannert
Journal:  J Bacteriol       Date:  2007-03-09       Impact factor: 3.490

7.  Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5.

Authors:  Kenolisa C Onwueme; Julian A Ferreras; John Buglino; Christopher D Lima; Luis E N Quadri
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-18       Impact factor: 11.205

8.  Plant surface lipid biosynthetic pathways and their utility for metabolic engineering of waxes and hydrocarbon biofuels.

Authors:  Reinhard Jetter; Ljerka Kunst
Journal:  Plant J       Date:  2008-05       Impact factor: 6.417

Review 9.  Bacterial acyltransferases as an alternative for lipase-catalyzed acylation for the production of oleochemicals and fuels.

Authors:  Tim Stöveken; Alexander Steinbüchel
Journal:  Angew Chem Int Ed Engl       Date:  2008       Impact factor: 15.336

10.  Crystal structure of carnitine acetyltransferase and implications for the catalytic mechanism and fatty acid transport.

Authors:  Gerwald Jogl; Liang Tong
Journal:  Cell       Date:  2003-01-10       Impact factor: 41.582
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  9 in total

Review 1.  Acyltransferases in bacteria.

Authors:  Annika Röttig; Alexander Steinbüchel
Journal:  Microbiol Mol Biol Rev       Date:  2013-06       Impact factor: 11.056

Review 2.  The production of wax esters in transgenic plants: 
towards a sustainable source of bio-lubricants.

Authors:  Frédéric Domergue; Magdalena Miklaszewska
Journal:  J Exp Bot       Date:  2022-05-13       Impact factor: 7.298

3.  Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity.

Authors:  Juan A Villa; Matilde Cabezas; Fernando de la Cruz; Gabriel Moncalián
Journal:  Appl Environ Microbiol       Date:  2013-12-02       Impact factor: 4.792

4.  Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters.

Authors:  Yang Zhang; Jie Peng; Huimin Zhao; Shuobo Shi
Journal:  Biotechnol Biofuels       Date:  2021-05-08       Impact factor: 6.040

5.  Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property.

Authors:  Hui Tao; Daoyi Guo; Yuchen Zhang; Zixin Deng; Tiangang Liu
Journal:  Biotechnol Biofuels       Date:  2015-06-24       Impact factor: 6.040

6.  Two Predicted Transmembrane Domains Exclude Very Long Chain Fatty acyl-CoAs from the Active Site of Mouse Wax Synthase.

Authors:  Steffen Kawelke; Ivo Feussner
Journal:  PLoS One       Date:  2015-12-29       Impact factor: 3.240

7.  High-level accumulation of oleyl oleate in plant seed oil by abundant supply of oleic acid substrates to efficient wax ester synthesis enzymes.

Authors:  Dan Yu; Ellen Hornung; Tim Iven; Ivo Feussner
Journal:  Biotechnol Biofuels       Date:  2018-03-01       Impact factor: 6.040

Review 8.  The Studies in Constructing Yeast Cell Factories for the Production of Fatty Acid Alkyl Esters.

Authors:  Yang Zhang; Xiao Guo; Huaiyi Yang; Shuobo Shi
Journal:  Front Bioeng Biotechnol       Date:  2022-01-11

9.  Uncovering the role of a positive selection site of wax ester synthase/diacylglycerol acyltransferase in two closely related Stipa species in wax ester synthesis under drought stress.

Authors:  Yunqiang Yang; Zhili Zhou; Yan Li; Yanqiu Lv; Danni Yang; Shihai Yang; Jianshuang Wu; Xiong Li; Zhijia Gu; Xudong Sun; Yongping Yang
Journal:  J Exp Bot       Date:  2020-07-06       Impact factor: 6.992
  9 in total

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