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Literature DB >> 2308578

The characteristics of purified HL60 tuftsin receptors.

Abstract

The purification and characteristics of purified HL60 tuftsin receptors are described. Purification was accomplished by affinity chromatography similar to that described earlier, wherein a tuftsin analog Thr-Lys-Pro-Pro-Arg, is covalently linked at the N alpha group to a solid support. The receptor consists presumably of two subunits approximately 66 KDa and 57 KDa. The dissociation constant of the receptor complex is 4.7 X 10(-8) M with 5 X 10(4) receptors per cell. It can form oligomers with an Mr of about 560 KDa suggesting an octomeric structure, assuming the same number of each subunit is associated.

Entities: Chemical

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Year: 1990 PMID： 2308578 DOI： 10.1007/bf00220722

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

29 in total

1. Solid-phase tuftsin (Thr-Lys-Pro-Arg) synthesis: deprotection and resin cleavage with trifluoromethane sulfonic acid.

Authors: M K Chaudhuri; V A Najjar
Journal: Anal Biochem Date: 1979-05 Impact factor: 3.365

2. Tuftsin (an Ig-associated tetrapeptide) triggers the immunogenic function of macrophages: implications for activation of programmed cells.

Authors: E Tzehoval; S Segal; Y Stabinsky; M Fridkin; Z Spirer; M Feldman
Journal: Proc Natl Acad Sci U S A Date: 1978-07 Impact factor: 11.205

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

4. The characteristics, isolation and synthesis of the phagocytosis stimulating peptide tuftsin.

Authors: K Nishioka; A Constantopoulos; P S Satoh; V A Najjar
Journal: Biochem Biophys Res Commun Date: 1972-04-14 Impact factor: 3.575

5. A new phagocytosis-stimulating tetrapeptide hormone, tuftsin, and its role in disease.

Authors: V A Najjar; A Constantopoulos
Journal: J Reticuloendothel Soc Date: 1972-08

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

Review 7. Tuftsin: a hormone-like tetrapeptide with antimicrobial and antitumor activities.

Authors: K Nishioka; A A Amoscato; G F Babcock
Journal: Life Sci Date: 1981-03-09 Impact factor: 5.037

8. Tuftsin-macrophage interaction: specific binding and augmentation of phagocytosis.

Authors: Z Bar-Shavit; Y Stabinsky; M Fridkin; R Goldman
Journal: J Cell Physiol Date: 1979-07 Impact factor: 6.384

9. Isolation and subunit composition of tuftsin receptor.

Authors: N J Bump; J Lee; M Wleklik; J Reichler; V A Najjar
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

10. Nuclear magnetic resonance studies on the structure of the tetrapeptide tuftsin, L-threonyl-L-lysyl-L-prolyl-L-arginine, and its pentapeptide analogue L-threonyl-L-lysyl-L-prolyl-L-prolyl-L-arginine.

Authors: M Blumenstein; P P Layne; V A Najjar
Journal: Biochemistry Date: 1979-11-13 Impact factor: 3.162

1 in total

1. Modulation of microglial/macrophage activation by macrophage inhibitory factor (TKP) or tuftsin (TKPR) attenuates the disease course of experimental autoimmune encephalomyelitis.

Authors: Madhuri Bhasin; Muzhou Wu; Stella E Tsirka
Journal: BMC Immunol Date: 2007-07-16 Impact factor: 3.615

1 in total