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Literature DB >> 23079923

Rational substrate and enzyme engineering of transketolase for aromatics.

Panwajee Payongsri¹, David Steadman, John Strafford, Andrew MacMurray, Helen C Hailes, Paul A Dalby.

Abstract

The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.

Entities: Chemical Gene Mutation

Mesh：

Substances：

Year: 2012 PMID： 23079923 DOI： 10.1039/c2ob25751c

Source DB: PubMed Journal: Org Biomol Chem ISSN： 1477-0520 Impact factor: 3.876

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Cited

11 in total

1. Droplet millifluidics for kinetic study of transketolase.

Authors: A Pinsolle; F Charmantray; L Hecquet; F Sarrazin
Journal: Biomicrofluidics Date: 2016-11-10 Impact factor: 2.800

2. Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes.

Authors: Pierre E Affaticati; Shao-Bo Dai; Panwajee Payongsri; Helen C Hailes; Kai Tittmann; Paul A Dalby
Journal: Sci Rep Date: 2016-10-21 Impact factor: 4.379

3. Two strategies to engineer flexible loops for improved enzyme thermostability.

Authors: Haoran Yu; Yihan Yan; Cheng Zhang; Paul A Dalby
Journal: Sci Rep Date: 2017-02-01 Impact factor: 4.379

4. Biotransformation of β-hydroxypyruvate and glycolaldehyde to l-erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase.

Authors: Yu-Chia Wei; Stephanie Braun-Galleani; Maria José Henríquez; Sahan Bandara; Darren Nesbeth
Journal: Biotechnol Prog Date: 2017-11-20

5. Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics.

Authors: Haoran Yu; Paul A Dalby
Journal: Proc Natl Acad Sci U S A Date: 2018-11-07 Impact factor: 11.205

10. A 'Split-Gene' Transketolase From the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization.

Authors: Paul James; Michail N Isupov; Simone Antonio De Rose; Christopher Sayer; Isobel S Cole; Jennifer A Littlechild
Journal: Front Microbiol Date: 2020-10-30 Impact factor: 5.640

Rational substrate and enzyme engineering of transketolase for aromatics.

1. Droplet millifluidics for kinetic study of transketolase.

2. Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes.

3. Two strategies to engineer flexible loops for improved enzyme thermostability.

4. Biotransformation of β-hydroxypyruvate and glycolaldehyde to l-erythrulose by Pichia pastoris strain GS115 overexpressing native transketolase.

5. Coupled molecular dynamics mediate long- and short-range epistasis between mutations that affect stability and aggregation kinetics.

6. Exploiting correlated molecular-dynamics networks to counteract enzyme activity-stability trade-off.

7. Microfluidic multi-input reactor for biocatalytic synthesis using transketolase.

Review 8. Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.

9. Oxalyl-CoA Decarboxylase Enables Nucleophilic One-Carbon Extension of Aldehydes to Chiral α-Hydroxy Acids.

10. A 'Split-Gene' Transketolase From the Hyper-Thermophilic Bacterium Carboxydothermus hydrogenoformans: Structure and Biochemical Characterization.