BACKGROUND: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. METHODS: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. RESULTS: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. CONCLUSIONS: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
BACKGROUND: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. METHODS: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. RESULTS: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. CONCLUSIONS: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
Authors: A J Bennett; K P Lesch; A Heils; J C Long; J G Lorenz; S E Shoaf; M Champoux; S J Suomi; M V Linnoila; J D Higley Journal: Mol Psychiatry Date: 2002 Impact factor: 15.992
Authors: Avshalom Caspi; Joseph McClay; Terrie E Moffitt; Jonathan Mill; Judy Martin; Ian W Craig; Alan Taylor; Richie Poulton Journal: Science Date: 2002-08-02 Impact factor: 47.728
Authors: Leslie A Lyons; David S Biller; Carolyn A Erdman; Monika J Lipinski; Amy E Young; Bruce A Roe; Baifang Qin; Robert A Grahn Journal: J Am Soc Nephrol Date: 2004-10 Impact factor: 10.121
Authors: Genesio M Karere; Erin L Kinnally; Jessica N Sanchez; Thomas R Famula; Leslie A Lyons; John P Capitanio Journal: Biol Psychiatry Date: 2008-12-21 Impact factor: 13.382
Authors: Mari S Golub; Edward P Hackett; Casey E Hogrefe; Csaba Leranth; John D Elsworth; Robert H Roth Journal: Dev Cogn Neurosci Date: 2017-05-01 Impact factor: 6.464