Literature DB >> 23076364

Expression of a keratinase (kerA) gene from Bacillus licheniformis in Escherichia coli and characterization of the recombinant enzymes.

Hong Hu1, Jun He, Bing Yu, Ping Zheng, Zhiqing Huang, Xiangbing Mao, Jie Yu, Guoquan Han, Daiwen Chen.   

Abstract

The gene kerA (1,047 bp) encoding the main keratinase from Bacillus licheniformis was cloned into two conventional vectors, pET30α and pET32α, and expressed in Escherichia coli. From SDS-PAGE analysis, the recombinant keratinases were 45 and 55 kDa. They had different optimal pH values (7.5 and 8.5) but the same optimum temperature of 50 °C. The recombinant keratinase produced in E. coli pET30α-kerA was more stable than that produced in E. coli pET32α-kerA, and retained approx. 70 % of its total enzyme activity after 30 min at 70 °C.

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Year:  2012        PMID： 23076364     DOI： 10.1007/s10529-012-1064-7

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  8 in total

Review 1.  Molecular strategies to increase keratinase production in heterologous expression systems for industrial applications.

Authors:  Radin Shafierul Radin Yahaya; Yahaya M Normi; Lai Yee Phang; Siti Aqlima Ahmad; Janna Ong Abdullah; Suriana Sabri
Journal:  Appl Microbiol Biotechnol       Date:  2021-05-03       Impact factor: 4.813

2.  Molecular and biochemical characterization of a thermostable keratinase from Bacillus altitudinis RBDV1.

Authors:  Vishakha A Pawar; Anil S Prajapati; Rekha C Akhani; Darshan H Patel; R B Subramanian
Journal:  3 Biotech       Date:  2018-02-02       Impact factor: 2.406

3.  Cloning and expression of a thermostable keratinase gene from Thermoactinomyces sp. YT06 in Escherichia coli and characterization of purified recombinant enzymes.

Authors:  Lin Wang; Ying Zhou; Ying Huang; Qishun Wei; Hongying Huang; Chengbao Guo
Journal:  World J Microbiol Biotechnol       Date:  2019-08-20       Impact factor: 3.312

4.  Expression and characterization of extreme alkaline, oxidation-resistant keratinase from Bacillus licheniformis in recombinant Bacillus subtilis WB600 expression system and its application in wool fiber processing.

Authors:  Baihong Liu; Juan Zhang; Ben Li; Xiangru Liao; Guocheng Du; Jian Chen
Journal:  World J Microbiol Biotechnol       Date:  2012-12-21       Impact factor: 3.312

5.  Expression of a β-mannosidase from Paenibacillus polymyxa A-8 in Escherichia coli and characterization of the recombinant enzyme.

Authors:  Xi Bai; Hong Hu; Huaping Chen; Quanbin Wei; Zeshen Yang; Qianming Huang
Journal:  PLoS One       Date:  2014-11-25       Impact factor: 3.240

6.  Production of Thermostable Organic Solvent Tolerant Keratinolytic Protease from Thermoactinomyces sp. RM4: IAA Production and Plant Growth Promotion.

Authors:  Amit Verma; Hukum Singh; Mohammad S Anwar; Shailendra Kumar; Mohammad W Ansari; Sanjeev Agrawal
Journal:  Front Microbiol       Date:  2016-08-05       Impact factor: 5.640

7.  Codon optimization significantly improves the expression level of a keratinase gene in Pichia pastoris.

Authors:  Hong Hu; Jie Gao; Jun He; Bing Yu; Ping Zheng; Zhiqing Huang; Xiangbing Mao; Jie Yu; Guoquan Han; Daiwen Chen
Journal:  PLoS One       Date:  2013-03-05       Impact factor: 3.240

Review 8.  Keratinases as Versatile Enzymatic Tools for Sustainable Development.

Authors:  Marcin Sypka; Iga Jodłowska; Aneta M Białkowska
Journal:  Biomolecules       Date:  2021-12-18
  8 in total

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