Literature DB >> 2307230

A pseudosubstrate peptide inhibits protein kinase C-mediated phosphorylation in permeabilized Rat-1 cells.

T Eichholtz1, J Alblas, M van Overveld, W Moolenaar, H Ploegh.   

Abstract

Activation of protein kinase C (PKC) in Rat-1 fibroblasts leads to rapid phosphorylation of an 80-kDa protein, a major substrate of PKC. Digitonin-permeabilized cells perfectly supported this early response. Introduction of a PKC pseudosubstrate peptide inhibited 80 kDa phosphorylation with an IC50 of 1 microM, while a control peptide had no effect. The results indicate that this semi-intact cell system can be used in combination with the inhibitory pseudosubstrate peptide to study the involvement of PKC in cellular processes.

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Year:  1990        PMID： 2307230     DOI： 10.1016/0014-5793(90)80657-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

Review 1.  Protein kinase C isoenzymes: divergence in signal transduction?

Authors:  H Hug; T F Sarre
Journal:  Biochem J       Date:  1993-04-15       Impact factor: 3.857

2.  Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.

Authors:  C P Wheeler-Jones; M P Learmonth; H Martin; A Aitken
Journal:  Biochem J       Date:  1996-04-01       Impact factor: 3.857

Review 3.  The use of permeabilized cells to assay protein phosphorylation and catecholamine release.

Authors:  C A Gonçalves; C Gottfried; P R Dunkley
Journal:  Neurochem Res       Date:  2000-06       Impact factor: 3.996

4.  Overexpression of atypical protein kinase C in HeLa cells facilitates macropinocytosis via Src activation.

Authors:  Ellen J Tisdale; Assia Shisheva; Cristina R Artalejo
Journal:  Cell Signal       Date:  2014-02-27       Impact factor: 4.315

5.  Protein phosphorylation in the regulation of insulin secretion: the use of site-directed inhibitory peptides in electrically permeabilised islets of Langerhans.

Authors:  H Basudev; P M Jones; S L Howell
Journal:  Acta Diabetol       Date:  1995-03       Impact factor: 4.280

Review 6.  Towards selective pharmacological modulation of protein kinase C--opportunities for the development of novel antineoplastic agents.

Authors:  A Gescher
Journal:  Br J Cancer       Date:  1992-07       Impact factor: 7.640
  6 in total

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