| Literature DB >> 23070776 |
Olivia M Merkel1, Leigh M Marsh, Holger Garn, Thomas Kissel.
Abstract
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected have so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein-expressing mice (EGFP mice) for the differentiation of transfected lung cell populations based on their antigen expression.Entities:
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Year: 2013 PMID： 23070776 PMCID： PMC3841064 DOI： 10.1007/978-1-62703-140-0_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745