OBJECTIVE: An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue. METHODS: Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO(4). Groups 3 and 4 were sensitized with 5% NiSO(4) and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates. RESULTS: A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP. DISCUSSION: Our results suggest that both NiSO(4) sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.
OBJECTIVE: An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue. METHODS: Female Wistar rats were divided into six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO(4). Groups 3 and 4 were sensitized with 5% NiSO(4) and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g), respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates. RESULTS: A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP. DISCUSSION: Our results suggest that both NiSO(4) sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.
Authors: Flávia S Fernandes; Gustavo S da Silva; Alexandre S Hilel; Ana C Carvalho; Karina V T Remor; Aline D Schlindwein; Luiz A Kanis; Daniel F Martins; Maicon R Kviecinski Journal: PLoS One Date: 2019-05-31 Impact factor: 3.240
Authors: Maicon Roberto Kviecinski; Isabela Machado Barbosa David; Flávia de Souza Fernandes; Marina Dos Reis Correa; Morgana Miranda Clarinda; Amanda Fernandes Freitas; Jane da Silva; Marta Gava; Simony Davet Müller; Drielly Florentino; Fabrícia Petronilho; Diego Moterle; Luiz Alberto Kanis; Rozangela Curi Pedrosa Journal: Pharm Biol Date: 2017-12 Impact factor: 3.503