Literature DB >> 2306877

Highly sensitive microplate-ELISA for angiotensin I using 3,3', 5,5'-tetramethylbenzidine.

F Suzuki1, S Yamashita, A Takahashi, M Ito, S Miyazaki, Y Nagata, Y Nakamura.   

Abstract

A microplate-ELISA was established for angiotensin (Ang) I using Ang I-peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3', 5,5'-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.

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Year:  1990        PMID： 2306877     DOI： 10.3109/10641969009074721

Source DB:  PubMed          Journal:  Clin Exp Hypertens A        ISSN： 0730-0077


  3 in total

1.  Aliskiren reduces the release of soluble (pro)renin receptor from human umbilical vein endothelial cells.

Authors:  Shinji Yamashita; Kazal Boron Biswas; A H M Nurun Nabi; Tsutomu Nakagawa; Fumiaki Suzuki; Akio Ebihara
Journal:  Biomed Rep       Date:  2018-07-05

2.  Escherichia coli-based production of recombinant ovine angiotensinogen and its characterization as a renin substrate.

Authors:  Shinji Yamashita; Naoya Shibata; Akiyoshi Boku-Ikeda; Erika Abe; Ayumi Inayama; Takashi Yamaguchi; Ayano Higuma; Kaoru Inagaki; Tomoyo Tsuyuzaki; Satoshi Iwamoto; Satoshi Ohno; Takashi Yokogawa; Kazuya Nishikawa; Kazal Boron Biswas; A H M Nurun Nabi; Tsutomu Nakagawa; Fumiaki Suzuki; Akio Ebihara
Journal:  BMC Biotechnol       Date:  2016-04-07       Impact factor: 2.563

3.  Establishing a novel assay system for measuring renin concentration using cost effective recombinant ovine angiotensinogen.

Authors:  Jobaida Akther; A H M Nurun Nabi; Satoshi Ohno; Takashi Yokogawa; Tsutomu Nakagawa; Fumiaki Suzuki; Akio Ebihara
Journal:  Heliyon       Date:  2019-04-04
  3 in total

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