| Literature DB >> 23065564 |
Abstract
Selenium derivatization of RNA is an important strategy for crystal structure determination and functional studies of noncoding RNAs and protein-RNA interactions. We describe here the synthesis of nucleoside 5'-(α-P-seleno)-triphosphate analogs (Se-NTPs) and their use in vitro transcription and purification of Se-derivatized RNA samples (phosphoroselenoate RNA, PSe-RNA).Entities:
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Year: 2012 PMID： 23065564 DOI： 10.1007/978-1-62703-113-4_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745