Manoj Goyal1, B P Nagori, D Sasmal. 1. Lachoo Memorial College of Science and Technology, Pharmacy Wing, Jodhpur 342008, Rajasthan, India. manojgoyal620@yahoo.com
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: [corrected] Euphorbia caducifolia is a Euphorbiaceae species native to Thar Desert of India, where latex of E. caducifolia (ECL) is used by the local inhabitants for treatment of bleeding wound, cutaneous eruption and other skin diseases. Looking to the medicinal importance of the plant, it was considered worthwhile to scientifically evaluate the ECL for the wound healing activity. MATERIAL AND METHODS: In vitro methods, clotting of platelet free plasma and angiogenesis in chick chorioallantoic membrane (CCM) were used for studying effect of ECL on clotting and angiogenesis. Excision and incision wounds model were used to study effect of ECL on wound contraction, tensile strength and hydroxyproline and DNA content. RESULTS: ECL treatment stimulates the blood vessel formation in CCM, at the all dose levels. 56.77, 74.48 and 78.09% increase in area of angiogenesis was observed in ECL 2.5, 5.0 and 10mg/ml treated groups respectively. The excised skin of ECL 5.0 and 10mg/ml treated animals found to have higher content of hydroxyproline and DNA, when compared with control group. An acceleration of the wound closure was observed with an enhanced number of fibroblasts and collagen content in ECL treated mice. CONCLUSION: Result of in vitro and in vivo experiments indicate that ECL exerts significant wound healing activity, demonstrating its relevant therapeutic potential.
ETHNOPHARMACOLOGICAL RELEVANCE: [corrected] Euphorbia caducifolia is a Euphorbiaceae species native to Thar Desert of India, where latex of E. caducifolia (ECL) is used by the local inhabitants for treatment of bleeding wound, cutaneous eruption and other skin diseases. Looking to the medicinal importance of the plant, it was considered worthwhile to scientifically evaluate the ECL for the wound healing activity. MATERIAL AND METHODS: In vitro methods, clotting of platelet free plasma and angiogenesis in chick chorioallantoic membrane (CCM) were used for studying effect of ECL on clotting and angiogenesis. Excision and incision wounds model were used to study effect of ECL on wound contraction, tensile strength and hydroxyproline and DNA content. RESULTS:ECL treatment stimulates the blood vessel formation in CCM, at the all dose levels. 56.77, 74.48 and 78.09% increase in area of angiogenesis was observed in ECL 2.5, 5.0 and 10mg/ml treated groups respectively. The excised skin of ECL 5.0 and 10mg/ml treated animals found to have higher content of hydroxyproline and DNA, when compared with control group. An acceleration of the wound closure was observed with an enhanced number of fibroblasts and collagen content in ECL treated mice. CONCLUSION: Result of in vitro and in vivo experiments indicate that ECL exerts significant wound healing activity, demonstrating its relevant therapeutic potential.
Authors: Luciane Madureira Almeida; Juliana Ferreira Floriano; Thuanne Pires Ribeiro; Lais Nogueira Magno; Lígia Souza Lima Silveira da Mota; Nei Peixoto; Fátima Mrué; Paulo Melo-Reis; Ruy de Souza Lino Junior; Carlos Frederico de Oliveira Graeff; Pablo José Gonçalves Journal: J Mater Sci Mater Med Date: 2014-06-29 Impact factor: 3.896
Authors: Lucas Fernando Sérgio Gushiken; Carlos Alberto Hussni; Jairo Kenupp Bastos; Ariane Leite Rozza; Fernando Pereira Beserra; Ana Júlia Vieira; Carlos Roberto Padovani; Marivane Lemos; Maurilio Polizello Junior; Jonas Joaquim Mangabeira da Silva; Rafael Henrique Nóbrega; Emanuel Ricardo Monteiro Martinez; Cláudia Helena Pellizzon Journal: Evid Based Complement Alternat Med Date: 2017-08-27 Impact factor: 2.629