| Literature DB >> 23063685 |
Floor A Harms1, Wilhelmina J Voorbeijtel, Sander I A Bodmer, Nicolaas J H Raat, Egbert G Mik.
Abstract
Progress in diagnosis and treatment of mitochondrial dysfunction in chronic and acute disease could greatly benefit from techniques for monitoring of mitochondrial function in vivo. In this study we demonstrate the feasibility of in vivo respirometry in skin. Mitochondrial oxygen measurements by means of oxygen-dependent delayed fluorescence of protoporphyrin IX are shown to provide a robust basis for measurement of local oxygen disappearance rate (ODR). The fundamental principles behind the technology are described, together with an analysis method for retrievel of respirometry data. The feasibility and reproducibility of this clinically useful approach are demonstrated in a series of rats.Entities:
Keywords: 5-aminolevulinic acid; ALA; Autoconsumption coefficient; DOI; Delayed fluorescence; Diffusive Oxygen Influx; Inflow coefficient of oxygen; Initial oxygen disappearance rate; K; Lifetime at zero oxygen; Maximal tissue oxygen consumption; Mean PO(2) before stop-flow; Measurement volume; MitoPO(2) at which oxygen consumption and influx are in equilibrium; Mitochondrial oxygen tension; NADH; NIRS; Near Infrared Spectroscopy; Nicotinamide Adenine Dinucleotide Hydrogen; OCM; ODC; ODR; Oxygen Consumption by the Measurement; Oxygen Disappearance Curve; Oxygen Disappearance Rate; Oxygen consumption; Oxygen disappearance rate; Oxygen-dependent quenching; P(0); P(50); P(eq); PO(2); PO(2) at which oxygen consumption is 1/2 V(max); Partial Oxygen Pressure; Phosphorescence quenching; PpIX-TSLT; Protoporphyrin IX; Protoporphyrin IX-Triplet State Lifetime Technique; Quenching constant; RDM; Rectangular Distribution Method; Tissue oxygenation; V(0); V(m); V(max); VO(2); Z; k(q); mitoPO(2); τ(0)
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Year: 2012 PMID： 23063685 DOI： 10.1016/j.mito.2012.10.005
Source DB: PubMed Journal: Mitochondrion ISSN： 1567-7249 Impact factor: 4.160