Juan P Ruiz1, Daniel Pelaez, Janice Dias, Noël M Ziebarth, Herman S Cheung. 1. Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, FL, USA ; Research Service and Geriatrics Research, Education, and Clinical Center, Veterans Affairs Medical Center, Miami, FL, USA.
Abstract
PURPOSE: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. METHODS: MSCs were synchronized to the G(0) stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 µM, 0.5 µM, and 1 µM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. RESULTS: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 µM and 1.0 µM concentrations only. CONCLUSIONS: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.
PURPOSE: To measure the elasticity of the nucleus and cytoplasm of human mesenchymal stem cells (MSCs) as well as changes brought about by exposure to nicotine in vitro. METHODS: MSCs were synchronized to the G(0) stage of the cell cycle through serum deprivation techniques. The cells were then treated with medium containing nicotine (0.1 µM, 0.5 µM, and 1 µM). Atomic force microscopy was then used to measure the Young's modulus of both the nucleus and cytoplasm of these cells. RESULTS: For both unsynchronized and synchronized cells, the nucleus was softer than the cytoplasm, although this difference was not found to be statistically significant. The nucleus of cells treated with nicotine was significantly stiffer than the control for all concentrations. The cytoplasm was significantly stiffer in nicotine-treated cells than in control cells for the 0.5 µM and 1.0 µM concentrations only. CONCLUSIONS: The results of this study could suggest that nicotine affects the biophysical properties of human MSCs in a dose-dependent manner, which may render the cells less responsive to mechanoinduction and other physical stimuli.
Authors: Wenxiao Pan; Erik Petersen; Ning Cai; Gang Ma; Jian Run Lee; Zhiqin Feng; Kin Liao; Kam Leong Journal: Conf Proc IEEE Eng Med Biol Soc Date: 2005
Authors: Y Wang; E F Pereira; A D Maus; N S Ostlie; D Navaneetham; S Lei; E X Albuquerque; B M Conti-Fine Journal: Mol Pharmacol Date: 2001-12 Impact factor: 4.436
Authors: Chenbo Dong; Michael L Kashon; David Lowry; Jonathan S Dordick; Steven H Reynolds; Yon Rojanasakul; Linda M Sargent; Cerasela Zoica Dinu Journal: Adv Healthc Mater Date: 2013-01-18 Impact factor: 9.933
Authors: Chenbo Dong; Reem Eldawud; Linda M Sargent; Michael L Kashon; David Lowry; Yon Rojanasakul; Cerasela Zoica Dinu Journal: J Mater Chem B Date: 2015 Impact factor: 6.331
Authors: Laurin Marie Pacheco; Lourdes Adriana Gomez; Janice Dias; Noel M Ziebarth; Guy A Howard; Paul C Schiller Journal: Aging (Albany NY) Date: 2014-12 Impact factor: 5.682
Authors: Tsz Kin Ng; Li Huang; Di Cao; Yolanda Wong-Ying Yip; Wai Ming Tsang; Gary Hin-Fai Yam; Chi Pui Pang; Herman S Cheung Journal: Sci Rep Date: 2015-01-16 Impact factor: 4.379