Separation of polyprenyl phosphate oligomerhomologues by reversed-phase ion-pair high-performance liquid chromatography.

A simple and rapid method of reversed-phase ion-pair high-performance liquid chromatography (HPLC) with UV-detection on a common C(18) column in an isocratic mode with the addition of tetrabutylammonium phosphate for the separation of polyprenyl phosphate oligomerhomologues has been developed. The method was successfully applied to assay the composition of polyprenyl phosphates obtained from polyprenols isolated from mulberry (Morus alba) leaves and fir (Abies sibirica) needles (the active components of veterinary drugs Gamapren and Phosprenyl correspondingly).