| Literature DB >> 23059551 |
Renée M van der Sluis1, Thijs van Montfort, Mireille Centlivre, Nick C T Schopman, Marion Cornelissen, Rogier W Sanders, Ben Berkhout, Rienk E Jeeninga, William A Paxton, Georgios Pollakis.
Abstract
The increasing diversity of HIV-1 isolates makes virus quantitation challenging, especially when diverse isolates co-circulate in a geographical area. Measuring the HIV-1 DNA levels in cells has become a valuable practical tool for fundamental and clinical research. A quantitative HIV-1 DNA assay was developed based on TaqMan(®) technology. Primers that target the highly conserved LTR region were designed to detect a broad array of HIV-1 variants, including viral isolates from many subtypes, with high sensitivity. Introduction of a pre-amplification step prior to the TaqMan(®) reaction allowed the specific amplification of fully reverse transcribed viral DNA. Execution of the pre-amplification step with a second primer set enables for the exclusive quantitation of the 2-LTR circular HIV-1 DNA form.Entities:
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Year: 2012 PMID： 23059551 DOI： 10.1016/j.jviromet.2012.09.019
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014