BACKGROUND: Collagen type I (Col-I) is a major component of the extracellular matrix and is important in wound healing processes. Several studies have shown that low-intensity laser irradiation (LILI) biostimulates Col-I synthesis both in vitro and in vivo. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model. MATERIALS AND METHODS: This study was performed on isolated human skin fibroblasts. Different cell models (normal and diabetic wounded) were used. Cells were irradiated with 5 J/cm(2) at a wavelength of 660 nm and incubated for 48 or 72 h. Nonirradiated cells (0 J/cm(2)) were used as controls. Cellular viability (Trypan blue exclusion test), morphology (bright-field microscopy), proliferation [VisionBlue™ quick cell proliferation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and Col-I (enzyme-linked immunoabsorbent assay) were assessed. RESULTS: Diabetic wounded cells irradiated with 5 J/cm(2) at 660 nm showed a significant increase in cell migration, viability, proliferation, and collagen content. CONCLUSIONS: This study shows that LILI stimulates Col-I synthesis in diabetic wound healing in vitro at 660 nm.
BACKGROUND: Collagen type I (Col-I) is a major component of the extracellular matrix and is important in wound healing processes. Several studies have shown that low-intensity laser irradiation (LILI) biostimulates Col-I synthesis both in vitro and in vivo. This study aimed to determine if LILI affects collagen production and related cellular responses in an in vitro diabetic wounded fibroblast model. MATERIALS AND METHODS: This study was performed on isolated human skin fibroblasts. Different cell models (normal and diabetic wounded) were used. Cells were irradiated with 5 J/cm(2) at a wavelength of 660 nm and incubated for 48 or 72 h. Nonirradiated cells (0 J/cm(2)) were used as controls. Cellular viability (Trypan blue exclusion test), morphology (bright-field microscopy), proliferation [VisionBlue™ quick cell proliferation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and Col-I (enzyme-linked immunoabsorbent assay) were assessed. RESULTS:Diabetic wounded cells irradiated with 5 J/cm(2) at 660 nm showed a significant increase in cell migration, viability, proliferation, and collagen content. CONCLUSIONS: This study shows that LILI stimulates Col-I synthesis in diabetic wound healing in vitro at 660 nm.
Authors: Aline Carla Teles de Sousa; Ítalo Bruno Paiva da Rocha; Ana Flávia Machado de Carvalho; Nayana Pinheiro Machado de Freitas Coelho; Maura Cristina Porto Feitosa; Esmeralda Maria Lustosa Barros; Emilia Angela Lo Schiavo Arisawa; Maria Rosilândia Lopes de Amorim Journal: J Lasers Med Sci Date: 2017-03-20
Authors: Franciane Barbieri Fiorio; Solange Almeida Dos Santos; Caroline Sobral de Melo Rambo; Camila Guerra Dalbosco; Andrey Jorge Serra; Brunno Lemes de Melo; Ernesto Cesar Pinto Leal-Junior; Paulo de Tarso Camillo de Carvalho Journal: Lasers Med Sci Date: 2017-07-05 Impact factor: 3.161