A method for determination of muscle fiber diameter using single fiber potential (SFP) analysis.

We have used computer simulation to study the relationship between the muscle fiber diameter and parameters: peak-to-peak amplitude and duration of the negative peak of the muscle fiber action potential. We found that the negative peak duration is useful in the determination of fiber diameter via the diameter dependence of conduction velocity. We have shown a direct link between the underlying physiology and the measurements characterizing single fiber potential. Using data from simulations, a graphical tool and an analytical method to estimate the muscle fiber diameter from the recorded action potential has been developed. The ability to quantify the fiber diameter can add significantly to the single fiber electromyography examination. It may help study of muscle fiber diameter variability and thus compliment the muscle biopsy studies.