Cloning, characterization and expression of estrogen receptor beta in the male half-smooth tongue sole, Cynoglossus semilaevis.

A full-length sequence encoding the estrogen receptor beta was isolated from half-smooth tongue sole, Cynoglossus semilaevis (hstsERβ) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends procedures. The hstsERβ cDNA clone was found to contain 1,791 nucleotides including an open reading frame that encodes 578 amino acids. The deduced hstsERβ protein consisted of six nuclear receptor-characteristic domains. Based on a phylogenetic analysis, the hstsERβ C and E domains are highly conserved compared to other fishes. The potential phosphorylation sites for PKC, CK-2 and PTK are also found in this protein. Highest amino acid identities were found for hstsERβ with common carp (Cyprinus carpio) ERβ (76 %) and Japanese flounder (Paralichthys olivaceus) ERβ (76 %). Tissue expression analysis confirmed that the hstsERβ was widely distributed and predominantly expressed in testis, brain and liver. Seasonal changes in the testis, brain and liver expression profiles of hstsERβ were examined by RT-PCR; the present results suggest that level of hstsERβ in brain increased to the highest then decreases with gonadal growth; whereas in the testis and liver, the hstsERβ mRNA level dropped to lowest then slightly increased.