Literature DB >> 23045084

Detection of biliverdin reductase activity.

Tian-Jun Huang1.   

Abstract

The conversion of biliverdin to the bile pigment bilirubin is catalyzed by biliverdin reductase, a cytosolic enzyme with two pH optima with different cofactors. The enzyme is assayed at pH 8.7 with NADPH as a cofactor and at 6.75 with NADH. The production of bilirubin is detected as described in this unit with a spectrophotometric assay. The enzyme can be qualitatively assayed by immunoblotting and changes in reductase isoform expression can be detected by two-dimensional electrophoresis.

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Year:  2002        PMID： 23045084     DOI： 10.1002/0471140856.tx0904s00

Source DB:  PubMed          Journal:  Curr Protoc Toxicol        ISSN： 1934-9254


  2 in total

1.  Biliverdin Reductase A Attenuates Hepatic Steatosis by Inhibition of Glycogen Synthase Kinase (GSK) 3β Phosphorylation of Serine 73 of Peroxisome Proliferator-activated Receptor (PPAR) α.

Authors:  Terry D Hinds; Katherine A Burns; Peter A Hosick; Lucien McBeth; Andrea Nestor-Kalinoski; Heather A Drummond; Abdulhadi A AlAmodi; Michael W Hankins; John P Vanden Heuvel; David E Stec
Journal:  J Biol Chem       Date:  2016-10-10       Impact factor: 5.157

2.  A Novel Fluorescence-Based Assay for the Measurement of Biliverdin Reductase Activity.

Authors:  Samuel O Adeosun; Kyle H Moore; David M Lang; Assumpta C Nwaneri; Terry D Hinds; David E Stec
Journal:  React Oxyg Species (Apex)       Date:  2018-01-01
  2 in total

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