| Literature DB >> 23045061 |
Abstract
Reduced glutathione, a thiol, is essential to the survival of the cells of most aerobic organisms. It is present intracellularly and provides protection from hydroperoxides and free radicals. This unit describes a continuous spectrophotometric assay for reductase activity: it follows the reduction of glutathione disulfide to reduced glutathione by monitoring the oxidation of NADPH as visualized by a decrease in absorbance at 340 nm.Entities:
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Year: 2001 PMID: 23045061 DOI: 10.1002/0471140856.tx0702s00
Source DB: PubMed Journal: Curr Protoc Toxicol ISSN: 1934-9254