Literature DB >> 23045057

HPLC-based assays for enzymes of glutathione biosynthesis.

C C White1, C J Krejsa, D L Eaton, T J Kavanagh.   

Abstract

Glutamate cysteine ligase and glutathione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.

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Year:  2001        PMID： 23045057     DOI： 10.1002/0471140856.tx0605s00

Source DB:  PubMed          Journal:  Curr Protoc Toxicol        ISSN： 1934-9254


  2 in total

1.  Mitochondrial-targeted peptide rapidly improves mitochondrial energetics and skeletal muscle performance in aged mice.

Authors:  Michael P Siegel; Shane E Kruse; Justin M Percival; Jorming Goh; Collin C White; Heather C Hopkins; Terrance J Kavanagh; Hazel H Szeto; Peter S Rabinovitch; David J Marcinek
Journal:  Aging Cell       Date:  2013-06-11       Impact factor: 9.304

2.  Improving mitochondrial function with SS-31 reverses age-related redox stress and improves exercise tolerance in aged mice.

Authors:  Matthew D Campbell; Jicheng Duan; Ashton T Samuelson; Matthew J Gaffrey; Gennifer E Merrihew; Jarrett D Egertson; Lu Wang; Theo K Bammler; Ronald J Moore; Collin C White; Terrance J Kavanagh; Joachim G Voss; Hazel H Szeto; Peter S Rabinovitch; Michael J MacCoss; Wei-Jun Qian; David J Marcinek
Journal:  Free Radic Biol Med       Date:  2018-12-28       Impact factor: 7.376
  2 in total

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