Pectic-oligosaccharides prepared by dynamic high-pressure microfluidization and their in vitro fermentation properties.

Pectic-oligosaccharides (POSs) were prepared from apple pectin by dynamic high-pressure microfluidization (DHPM). Operating under selected conditions (pectin concentration 1.84%, solution temperature 63 °C, DHPM pressure 155MPa and number of cycles 6 passes), 32.92% of the pectin was converted into POS. The resulting POS contains 29.56% galacturonic acid and 58.53% neutral sugars. The prebiotic properties of POS were then evaluated using a fecal batch culture fermentation. The POS increased the number of Bifidobacteria and Lactobacilli, and produced a higher concentration of acetic, lactic, and propionic acid than their parent pectin. Furthermore, POS decreased the number of Bacteroides and Clostridia while their parent pectin increased them. Moreover, the effects of POS on the growth of these bacteria and production of short-chain fatty acids are comparable to those of the most studied prebiotic, fructooligosaccharide. These results indicated that the POS prepared by DHPM has a potential to be an effective prebiotic.