| Literature DB >> 2304369 |
G M Anderson1, I N Mefford, T J Tolliver, M A Riddle, D M Ocame, J F Leckman, D J Cohen.
Abstract
An inter-laboratory comparison study was carried out in order to ascertain mean levels of serotonin (5-HT) in human lumbar cerebrospinal fluid (CSF). Analyses were performed using high performance liquid chromatography (HPLC) coupled with either electrochemical (LC-EC) or fluorometric (LC-F) detection. With the detection limits obtained (7-8 pg/ml for LC-EC, 7-15 pg/ml for LC-F) 5-HT was not usually detected in human lumbar CSF. The findings indicate that the true mean concentration of CSF 5-HT is less than 10 pg/ml. This upper limit is substantially lower than all previous reports of 5-HT concentrations in normal human lumbar CSF. The extremely low concentrations of 5-HT present in CSF make it unlikely that CSF 5-HT will be of clinical utility in assessing central serotonergic function.Entities:
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Year: 1990 PMID： 2304369 DOI： 10.1016/0024-3205(90)90030-u
Source DB: PubMed Journal: Life Sci ISSN： 0024-3205 Impact factor: 5.037