Literature DB >> 23042078

qCMA: a desktop application for quantitative collective cell migration analysis.

Amit Zeisel1, Assif Yitzhaky, Cindy Koerner, Mattia Lauriola, Hadas Cohen-Dvashi, Wolfgang J Köstler, Yosef Yarden, Stefan Wiemann, Eytan Domany.   

Abstract

Collective migration is an important cellular trait, which is intensely studied by both basic and translational researchers. Investigation of the underlying mechanisms necessitates high-throughput assays and computational algorithms capable of generating reproducible quantitative measurements of cell migration. We present a desktop tool that can be used easily by any researcher, to quantify both fluorescent and phase-contrast images produced in the course of commonly used gap closure ("scratch," "wound healing") collective migration assays. The software has a simple graphical interface that allows the user to tune the relevant parameters and process large numbers of images (or movies). The output contains segmented images and the numerical values inferred from them, allowing easy quantitative analysis of the results.

Mesh：

Year:  2012        PMID： 23042078     DOI： 10.1177/1087057112461940

Source DB:  PubMed          Journal:  J Biomol Screen        ISSN： 1087-0571


  6 in total

1.  Quantifying Intracellular Particle Flows by DIC Object Tracking.

Authors:  Anushree R Chaphalkar; Yash K Jawale; Dhruv Khatri; Chaitanya A Athale
Journal:  Biophys J       Date:  2021-01-11       Impact factor: 4.033

2.  Diurnal suppression of EGFR signalling by glucocorticoids and implications for tumour progression and treatment.

Authors:  Mattia Lauriola; Yehoshua Enuka; Amit Zeisel; Gabriele D'Uva; Lee Roth; Michal Sharon-Sevilla; Moshit Lindzen; Kirti Sharma; Nava Nevo; Morris Feldman; Silvia Carvalho; Hadas Cohen-Dvashi; Merav Kedmi; Nir Ben-Chetrit; Alon Chen; Rossella Solmi; Stefan Wiemann; Fernando Schmitt; Eytan Domany; Yosef Yarden
Journal:  Nat Commun       Date:  2014-10-03       Impact factor: 14.919

3.  Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone.

Authors:  Yehoshua Enuka; Morris E Feldman; Animesh Chowdhury; Swati Srivastava; Moshit Lindzen; Aldema Sas-Chen; Renaud Massart; David Cheishvili; Matthew J Suderman; Yoav Zaltsman; Chiara A Mazza; Kirti Shukla; Cindy Körner; Noa Furth; Mattia Lauriola; Moshe Oren; Stefan Wiemann; Moshe Szyf; Yosef Yarden
Journal:  Nucleic Acids Res       Date:  2017-12-15       Impact factor: 16.971

4.  Aeroallergen Der p 2 promotes motility of human non-small cell lung cancer cells via toll-like receptor-mediated up-regulation of urokinase-type plasminogen activator and integrin/focal adhesion kinase signaling.

Authors:  Chun-Hsiang Lin; Hui-Han Lin; Cheng-Yi Kuo; Shao-Hsuan Kao
Journal:  Oncotarget       Date:  2017-02-14

5.  Independent of ErbB1 gene copy number, EGF stimulates migration but is not associated with cell proliferation in non-small cell lung cancer.

Authors:  Camila Lauand; Paula Rezende-Teixeira; Beatriz Araújo Cortez; Evandro Luís de Oliveira Niero; Gláucia Maria Machado-Santelli
Journal:  Cancer Cell Int       Date:  2013-04-30       Impact factor: 5.722

6.  Epidermal growth-factor-induced transcript isoform variation drives mammary cell migration.

Authors:  Wolfgang J Köstler; Amit Zeisel; Cindy Körner; Jonathan M Tsai; Jasmine Jacob-Hirsch; Nir Ben-Chetrit; Kirti Sharma; Hadas Cohen-Dvashi; Assif Yitzhaky; Eric Lader; Ulrich Tschulena; Gideon Rechavi; Eytan Domany; Stefan Wiemann; Yosef Yarden
Journal:  PLoS One       Date:  2013-12-06       Impact factor: 3.240
  6 in total

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