Literature DB >> 23041020

Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.

V M Corman1, I Eckerle, T Bleicker, A Zaki, O Landt, M Eschbach-Bludau, S van Boheemen, R Gopal, M Ballhause, T M Bestebroer, D Muth, M A Müller, J F Drexler, M Zambon, A D Osterhaus, R M Fouchier, C Drosten.   

Abstract

We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.

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Year:  2012        PMID： 23041020     DOI： 10.2807/ese.17.39.20285-en

Source DB:  PubMed          Journal:  Euro Surveill        ISSN： 1025-496X


  302 in total

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4.  Blocking of exchange proteins directly activated by cAMP leads to reduced replication of Middle East respiratory syndrome coronavirus.

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5.  Asymptomatic Middle East respiratory syndrome coronavirus infection in rabbits.

Authors:  Bart L Haagmans; Judith M A van den Brand; Lisette B Provacia; V Stalin Raj; Koert J Stittelaar; Sarah Getu; Leon de Waal; Theo M Bestebroer; Geert van Amerongen; Georges M G M Verjans; Ron A M Fouchier; Saskia L Smits; Thijs Kuiken; Albert D M E Osterhaus
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7.  Link of a ubiquitous human coronavirus to dromedary camels.

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10.  Human cell tropism and innate immune system interactions of human respiratory coronavirus EMC compared to those of severe acute respiratory syndrome coronavirus.

Authors:  Florian Zielecki; Michaela Weber; Markus Eickmann; Larissa Spiegelberg; Ali Moh Zaki; Mikhail Matrosovich; Stephan Becker; Friedemann Weber
Journal:  J Virol       Date:  2013-02-28       Impact factor: 5.103
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