Literature DB >> 23037931

A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge.

Salifu Seidu-Larry1, Bettina Krieg, Markus Hirsch, Mark Helm, Olwen Domingo.   

Abstract

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

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Year:  2012        PMID： 23037931     DOI： 10.1039/c2cc34015a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  7 in total

1.  Live cell imaging of duplex siRNA intracellular trafficking.

Authors:  Markus Hirsch; Mark Helm
Journal:  Nucleic Acids Res       Date:  2015-04-13       Impact factor: 16.971

2.  Intermolecular 'cross-torque': the N4-cytosine propargyl residue is rotated to the 'CH'-edge as a result of Watson-Crick interaction.

Authors:  Olwen Domingo; Isabell Hellmuth; Andres Jäschke; Christoph Kreutz; Mark Helm
Journal:  Nucleic Acids Res       Date:  2015-04-30       Impact factor: 16.971

3.  New Techniques to Assess In Vitro Release of siRNA from Nanoscale Polyplexes.

Authors:  Bettina Krieg; Markus Hirsch; Erik Scholz; Lutz Nuhn; Ilja Tabujew; Heiko Bauer; Sandra Decker; Andriy Khobta; Manfred Schmidt; Wolfgang Tremel; Rudolf Zentel; Kalina Peneva; Kaloian Koynov; A James Mason; Mark Helm
Journal:  Pharm Res       Date:  2014-12-09       Impact factor: 4.200

4.  Site-specific dual-color labeling of long RNAs for single-molecule spectroscopy.

Authors:  Meng Zhao; Fabio D Steffen; Richard Börner; Michelle F Schaffer; Roland K O Sigel; Eva Freisinger
Journal:  Nucleic Acids Res       Date:  2018-02-16       Impact factor: 16.971

5.  Efficient access to 3'-terminal azide-modified RNA for inverse click-labeling patterns.

Authors:  Tobias Santner; Markus Hartl; Klaus Bister; Ronald Micura
Journal:  Bioconjug Chem       Date:  2013-12-20       Impact factor: 4.774

6.  Bioconjugation of Small Molecules to RNA Impedes Its Recognition by Toll-Like Receptor 7.

Authors:  Isabell Hellmuth; Isabel Freund; Janine Schlöder; Salifu Seidu-Larry; Kathrin Thüring; Kaouthar Slama; Jens Langhanki; Stefka Kaloyanova; Tatjana Eigenbrod; Matthias Krumb; Sandra Röhm; Kalina Peneva; Till Opatz; Helmut Jonuleit; Alexander H Dalpke; Mark Helm
Journal:  Front Immunol       Date:  2017-03-24       Impact factor: 7.561

7.  A novel route for preparing 5' cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry.

Authors:  Sylwia Walczak; Anna Nowicka; Dorota Kubacka; Kaja Fac; Przemyslaw Wanat; Seweryn Mroczek; Joanna Kowalska; Jacek Jemielity
Journal:  Chem Sci       Date:  2016-08-16       Impact factor: 9.825
  7 in total

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