Literature DB >> 23037915

Back-scattering interferometry: an ultrasensitive method for the unperturbed detection of acetylcholinesterase-inhibitor interactions.

Gabrielle L Haddad1, Sherri C Young, Ned D Heindel, Darryl J Bornhop, Robert A Flowers.   

Abstract

A series of inhibitors of acetylcholinesterase (AChE) have been screened by back-scattering interferometry (BSI). Enzyme levels as low as 100 pM (22,000 molecules of AChE) can be detected. This method can be used to screen for mixed AChE inhibitors, agents that have shown high efficacy against Alzheimer's disease, by detecting dual-binding interactions. E = enzyme, I = inhibitor, S = substrate.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 23037915      PMCID： PMC3687211          DOI： 10.1002/anie.201203640

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  52 in total

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2.  Free-solution, label-free molecular interactions studied by back-scattering interferometry.

Authors:  Darryl J Bornhop; Joey C Latham; Amanda Kussrow; Dmitry A Markov; Richard D Jones; Henrik S Sørensen
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3.  Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding.

Authors:  Yves Bourne; Zoran Radic; Gerlind Sulzenbacher; Esther Kim; Palmer Taylor; Pascale Marchot
Journal:  J Biol Chem       Date:  2006-07-12       Impact factor: 5.157

Review 4.  Fluoroimmunoassays and immunofluorometric assays.

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5.  A structural motif of acetylcholinesterase that promotes amyloid beta-peptide fibril formation.

Authors:  G V De Ferrari; M A Canales; I Shin; L M Weiner; I Silman; N C Inestrosa
Journal:  Biochemistry       Date:  2001-09-04       Impact factor: 3.162

Review 6.  Targeting beta-amyloid pathogenesis through acetylcholinesterase inhibitors.

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7.  Limitation of the Ellman method: cholinesterase activity measurement in the presence of oximes.

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8.  6-Coumarin diazonium salt: a specific affinity label of the Torpedo acetylcholinesterase peripheral site.

Authors:  I Schalk; L Ehret-Sabatier; Y Le Feuvre; S Bon; J Massoulie; M Goeldner
Journal:  Mol Pharmacol       Date:  1995-12       Impact factor: 4.436

9.  A fluorescence "turn-on" ensemble for acetylcholinesterase activity assay and inhibitor screening.

Authors:  Lihua Peng; Guanxin Zhang; Deqing Zhang; Junfeng Xiang; Rui Zhao; Yilin Wang; Daoben Zhu
Journal:  Org Lett       Date:  2009-09-03       Impact factor: 6.005

10.  Binding of 125I-fasciculin to rat brain acetylcholinesterase. The complex still binds diisopropyl fluorophosphate.

Authors:  P Marchot; A Khélif; Y H Ji; P Mansuelle; P E Bougis
Journal:  J Biol Chem       Date:  1993-06-15       Impact factor: 5.157
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  3 in total

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Journal:  ChemistryOpen       Date:  2022-02-24       Impact factor: 2.630

2.  Label-free quantification of calcium-sensor targeting to photoreceptor guanylate cyclase and rhodopsin kinase by backscattering interferometry.

Authors:  Stefan Sulmann; Amanda Kussrow; Darryl J Bornhop; Karl-Wilhelm Koch
Journal:  Sci Rep       Date:  2017-03-31       Impact factor: 4.379

3.  Rhodium-Catalyzed C-H Methylation and Alkylation Reactions by Carbene-Transfer Reactions.

Authors:  Claire Empel; Sripati Jana; Tim Langletz; Rene M Koenigs
Journal:  Chemistry       Date:  2022-02-02       Impact factor: 5.020
  3 in total

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