Literature DB >> 23035189

Recovery of Bordetella pertussis from PCR-positive nasopharyngeal samples is dependent on bacterial load.

Didrik F Vestrheim1, Martin Steinbakk, Martha L Bjørnstad, Amir Moghaddam, Nils Reinton, Mette L Dahl, Nils Grude, Per Sandven.   

Abstract

Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to samples with a high bacterial load.

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Year:  2012        PMID： 23035189      PMCID： PMC3502974          DOI： 10.1128/JCM.01553-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Prolonged survival of Bordetella pertussis in a simple buffer after nasopharyngeal secretion aspiration.

Authors:  S Halperin; A Kasina; M Swift
Journal:  Can J Microbiol       Date:  1992-11       Impact factor: 2.419

Review 2.  Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.

Authors:  Michael Loeffelholz
Journal:  J Clin Microbiol       Date:  2012-03-21       Impact factor: 5.948

3.  A multiplex PCR for detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, and Bordetella pertussis in clinical specimens.

Authors:  Erin A McDonough; Christopher P Barrozo; Kevin L Russell; David Metzgar
Journal:  Mol Cell Probes       Date:  2005-10       Impact factor: 2.365

4.  Changes in genetic diversity of the Bordetella pertussis population in the United Kingdom between 1920 and 2006 reflect vaccination coverage and emergence of a single dominant clonal type.

Authors:  David J Litt; Shona E Neal; Norman K Fry
Journal:  J Clin Microbiol       Date:  2009-01-21       Impact factor: 5.948

5.  Enrichment medium for the isolation of Bordetella.

Authors:  J Regan; F Lowe
Journal:  J Clin Microbiol       Date:  1977-09       Impact factor: 5.948

6.  Short-lived immunity against pertussis, age-specific routes of transmission, and the utility of a teenage booster vaccine.

Authors:  Jennie S Lavine; Ottar N Bjørnstad; Birgitte Freiesleben de Blasio; Jann Storsaeter
Journal:  Vaccine       Date:  2011-11-26       Impact factor: 3.641

Review 7.  The diagnosis of pertussis: which method to choose?

Authors:  Asma Zouari; Hanen Smaoui; Amel Kechrid
Journal:  Crit Rev Microbiol       Date:  2011-11-21       Impact factor: 7.624

8.  Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples.

Authors:  Katrin Kösters; Marion Riffelmann; Carl Heinz Wirsing VON König
Journal:  J Med Microbiol       Date:  2001-05       Impact factor: 2.472

Review 9.  Bordetella pertussis and vaccination: the persistence of a genetically monomorphic pathogen.

Authors:  Frits R Mooi
Journal:  Infect Genet Evol       Date:  2009-10-30       Impact factor: 3.342
  9 in total
  5 in total

Review 1.  Laboratory Diagnosis of Pertussis.

Authors:  Anneke van der Zee; Joop F P Schellekens; Frits R Mooi
Journal:  Clin Microbiol Rev       Date:  2015-10       Impact factor: 26.132

2.  Correlation of Real Time PCR Cycle Threshold Cut-Off with Bordetella pertussis Clinical Severity.

Authors:  Shelly Bolotin; Shelley L Deeks; Alex Marchand-Austin; Heather Rilkoff; Vica Dang; Ryan Walton; Ahmed Hashim; David Farrell; Natasha S Crowcroft
Journal:  PLoS One       Date:  2015-07-17       Impact factor: 3.240

3.  Epidemiology of pertussis in Denmark, 1995 to 2013.

Authors:  Tine Dalby; Peter Henrik Andersen; Steen Hoffmann
Journal:  Euro Surveill       Date:  2016-09-08

4.  Improving specificity of Bordetella pertussis detection using a four target real-time PCR.

Authors:  Helena Martini; Liselot Detemmerman; Oriane Soetens; Erlangga Yusuf; Denis Piérard
Journal:  PLoS One       Date:  2017-04-12       Impact factor: 3.240

5.  Isolate-Based Surveillance of Bordetella pertussis, Austria, 2018-2020.

Authors:  Adriana Cabal; Daniela Schmid; Markus Hell; Ali Chakeri; Elisabeth Mustafa-Korninger; Alexandra Wojna; Anna Stöger; Johannes Möst; Eva Leitner; Patrick Hyden; Thomas Rattei; Adele Habington; Ursula Wiedermann; Franz Allerberger; Werner Ruppitsch
Journal:  Emerg Infect Dis       Date:  2021-03       Impact factor: 6.883
  5 in total

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