PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
Authors: Caroline Pereira Santos; Andreo Fernando Aguiar; Ines Cristina Giometti; Thaoan Bruno Mariano; Carlos Eduardo Assumpção de Freitas; Gisele Alborghetti Nai; Selma Zambelli de Freitas; Maeli Dal Pai-Silva; Francis Lopes Pacagnelli Journal: Lasers Med Sci Date: 2018-01-15 Impact factor: 3.161
Authors: Natalia S Carvalho; Antonio F Moron; Ramkumar Menon; Sergio Cavalheiro; Mauricio M Barbosa; Herbene J Milani; Marcia M Ishigai Journal: Exp Ther Med Date: 2017-08-21 Impact factor: 2.447
Authors: Ashley N Leberfinger; Monika Hospodiuk; Abdon Pena-Francesch; Bugra Ayan; Veli Ozbolat; Srinivas V Koduru; Ibrahim T Ozbolat; Melik C Demirel; Dino J Ravnic Journal: Plast Reconstr Surg Glob Open Date: 2018-08-07