Gustavo Zapata1, Lourdes Racca, Julia Tau, Alejandro Berra. 1. Laboratorio de Investigaciones Oculares, Departamento de Patología, Facultad de Medicina, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. zapatagu@fcv.unlp.edu.ar
Abstract
PURPOSE: To evaluate and compare the efficacy of rapamycin used topically in a mouse model of herpetic stromal keratitis. METHODS: The corneas were infected with herpes simplex virus type-1 strain KOS. Animals were divided into: control (CG), rapamycin (RAPA), cyclosporine (CsA), and dexamethasone (DEXA). The evolution of the disease was assessed clinically and histologically. RESULTS: On day 10 postinfection (pi), the RAPA group showed only a significantly lower angiogenic development than the CG. On day 14 pi, the treated groups had significantly lower scores for angiogenesis and necrosis than the CG. Also, on day 14 pi, the RAPA and DEXA groups showed significantly lower histopathological scores compared to the CG. CONCLUSIONS: The topical application of 0.05% rapamycin showed greater efficacy than 0.5% cyclosporine and similar efficacy to 0.1% dexamethasone to minimize the immuno-inflammatory process. Also, rapamycin showed early inhibition of the formation of new vessels.
PURPOSE: To evaluate and compare the efficacy of rapamycin used topically in a mouse model of herpetic stromal keratitis. METHODS: The corneas were infected with herpes simplex virus type-1 strain KOS. Animals were divided into: control (CG), rapamycin (RAPA), cyclosporine (CsA), and dexamethasone (DEXA). The evolution of the disease was assessed clinically and histologically. RESULTS: On day 10 postinfection (pi), the RAPA group showed only a significantly lower angiogenic development than the CG. On day 14 pi, the treated groups had significantly lower scores for angiogenesis and necrosis than the CG. Also, on day 14 pi, the RAPA and DEXA groups showed significantly lower histopathological scores compared to the CG. CONCLUSIONS: The topical application of 0.05% rapamycin showed greater efficacy than 0.5% cyclosporine and similar efficacy to 0.1% dexamethasone to minimize the immuno-inflammatory process. Also, rapamycin showed early inhibition of the formation of new vessels.
Authors: Goran Petrovski; Kata Pasztor; Laszlo Orosz; Reka Albert; Edina Mencel; Morten C Moe; Kai Kaarniranta; Andrea Facsko; Klara Megyeri Journal: J Biosci Date: 2014-09 Impact factor: 1.826
Authors: Sanaz Gidfar; Farnoud Y Milani; Behrad Y Milani; Xiang Shen; Medi Eslani; Ilham Putra; Michael J Huvard; Hossein Sagha; Ali R Djalilian Journal: Sci Rep Date: 2017-01-05 Impact factor: 4.379
Authors: Victor G Sendra; Julia Tau; Gustavo Zapata; Romina M Lasagni Vitar; Eduardo Illian; Pablo Chiaradía; Alejandro Berra Journal: Front Immunol Date: 2021-02-25 Impact factor: 7.561