Literature DB >> 23024407

Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India.

S Parthiban1, Hirak Kumar Mukhopadhyay, D Panneer, P X Antony, R M Pillai.   

Abstract

A total of 128 faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis in and around Puducherry, South India. Eighteen clinical samples, showing high HA titre of 1:512 and above and positivity by polymerase chain reaction (PCR) with CPV-2ab primers, were subjected to virus isolation in CRFK cell line. Of the 18 samples processed, 3 samples (16.6%) were positive for CPV and were confirmed by haemagglutination, dot-ELISA, and IFAT. The three cell culture isolates were characterized as CPV-2b types by multiplex PCR as well as by monoclonal antibody typing.

Entities:  

Keywords:  CPV; CRFK; Cell culture; Dot-ELISA; HA; IFAT; Monoclonal typing; PCR

Year:  2011        PMID： 23024407      PMCID： PMC3209934          DOI： 10.1007/s12088-011-0105-6

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


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